Overexpressed LRIG3 gene ameliorates prostate cancer through suppression of cell invasion and migration

Prostate cancer (PC) is one of the most common human malignancies, which has been steadily rising among males in many countries. The leucine-rich repeats and immunoglobulin-like domains (LRIG) genes were applied in the prognosis of different types of cancers, including PC. In that case, this study is supposed to investigate the effects of LRIG3 on PC cells. LRIG3 was overexpressed or under-expressed by the rAd and siRNA to determine the effect of LRIG3 on PC and explore the possible mechanism. The mRNA and protein levels of LRIG3 were tested by RT-qPCR and western blot analysis. Cell apoptosis, viability, migration and invasion were measured using MTT assay, flow cytometry and Transwell assay respectively. We observed that LRIG3 mRNA and protein levels were decreased in PC-3 cells. PC-3 cells treated with rAd-LRIG3 exhibited significantly increased cell apoptosis while inhibited viability, adhesion, migration and invasion. However, C4-2 cells treated with siRNA-LRIG3 showed reciprocal results. In conclusion, these data of the current study indicated that overexpressed LRIG3 gene expression might inhibit the viability, adhesion, invasion and migration and promote the apoptosis of PC cells. rAd-mediated LRIG3 may facilitate a novel aspect of the treatment of patients suffering from with PC.