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Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
The nature of photoinduced intermolecular charger transfer in fluorescence resonance energy transfer
Huan Zong1, Xijiao Mu1, Jiangcai Wang1
1Beijing Advanced Innovation Center for Materials Genome Engineering, Beijing Key Laboratory for Magneto-Photoelectrical Composite and Interface Science, Center for Green Innovation, School of Mathematics and Physics, University of Science and Technology Beijing, Beijing 100083, People's Republic of China.
Abstract:
In this paper, we report time resolved fluorescence resonance energy transfer (FRET) using femtosecond ultrafast transient absorption spectroscopy. The lifetimes of FRET are strongly dependent on the molecular concentration and ratio of donor and acceptor. Also, in the FRET, photoinduced intermolecular charge transfer (PICT) is also investigated theoretically. The driving force for PICT in FRET system equals the reorganization energy, which gives barrier-less charge transfer according to Marcus theory. The rates of PICT in the FRET system can be estimated with our simplified Marcus equation. Our results of PICT in FRET system provide a new efficient way for the charge transfer in donor-acceptor system.
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