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Isolation of Chavibetol and Methyleugenol from Essential Oil of Pimenta pseudocaryophyllus by High Performance Liquid
Edenilson Dos Santos Niculau1,2, Leandro do Prado Ribeiro3, Thiago Felipe Ansante4
1Departamento de Química, Universidade Federal de São Carlos (DQ/UFSCar)-Rod. Washington Luís, Km 235, São Carlos CEP 13565-905, SP, Brazil. enicolau@uft.edu.br.
Abstract:
A high performance liquid chromatography (HPLC) method was developed for the simultaneous isolation, on a semi-preparative scale, of chavibetol and methyleugenol from the crude essential oil of P. pseudocaryophyllus leaves. The purity of the isolated compounds and their quantifications were developed using GC/FID. Chavibetol was isolated with high purity (98.7%) and mass recovery (94.6%). The mass recovery (86.4%) and purity (85.3%) of methyleugenol were lower than those of chavibetol. Both compounds were identified on the basis of spectral analysis. The results suggest that the method can provide chavibetol with high purity, mass recovery, and productivity from crude essential, which will be used in bioassays against stored insect pests.
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