Related Experiment Video

Updated: Feb 2, 2026

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model
08:53

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model

Published on: January 1, 2017

9.8K

Decreased microRNA-182-5p helps alendronate promote osteoblast proliferation and differentiation in osteoporosis via

Bao-Long Pan1, Zong-Wu Tong2, Shu-De Li3

  • 1Department of Laboratory, People's Hospital of Yuxi City, Yuxi 653100, P.R. China.

Bioscience Reports
|November 11, 2018
PubMed

Insights

Down-regulated microRNA-182-5p (miR-182-5p) enhances osteoblast activity in osteoporosis by targeting ADCY6 via the Rap1/MAPK pathway. This suggests miR-182-5p as a potential therapeutic target for bone diseases.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Bone Biology

Background:

  • Osteoporosis (OP) is a significant health concern causing bone fragility.
  • MicroRNAs (miRNAs) play crucial roles in bone endocrinology.
  • Understanding miRNA function is vital for developing treatments for bone diseases.

Purpose of the Study:

  • To investigate the role of microRNA-182-5p (miR-182-5p) in osteoblast proliferation and differentiation in alendronate (ALN)-treated osteoporosis (OP) rats.
  • To explore the targeting of adenylyl cyclase isoform 6 (ADCY6) by miR-182-5p and its effect on the Rap1/mitogen-activated protein kinase (MAPK) signaling pathway.

Main Methods:

  • Established rat models of osteoporosis and administered alendronate (ALN) treatment.
  • Quantified expression of miR-182-5p, ADCY6, and Rap1/MAPK pathway genes.
  • Utilized osteoblast transfection with miR-182-5p inhibitor/mimic, si-ADCY6, and U0126 (MAPK inhibitor).
  • Assessed cell proliferation, apoptosis, and differentiation markers (ALP, BGP, COL I).

Main Results:

  • Alendronate (ALN) treatment alleviated osteoporosis symptoms.
  • miR-182-5p was found to negatively target ADCY6, thereby inhibiting the Rap1/MAPK signaling pathway.
  • Down-regulation of miR-182-5p increased osteoblast proliferation and differentiation while reducing apoptosis, evidenced by decreased ALP, BGP, and COL I expression.

Conclusions:

  • Down-regulated miR-182-5p promotes osteoblast proliferation and differentiation in OP rats.
  • This effect is mediated through the activation of the Rap1/MAPK signaling pathway by up-regulating ADCY6.
  • miR-182-5p represents a potential novel therapeutic target for osteoporosis treatment.
Keywords:
ADCY6AlendronateMicroRNA-182OsteoblastsOsteoporosisRap1/MAPK signaling pathway

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.8K
Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation06:55

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Published on: July 17, 2019

15.4K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.0K
Decreasing Function01:27

Decreasing Function

A decreasing function describes a relationship where the output consistently declines as the input increases. This means that for any two input values, if one is greater than the other, the corresponding output is smaller. Mathematically, a function f is decreasing on an interval I if for every x1 < x2​ in I, f (x1) > f (x2). This type of behavior is visually identified on a graph that slopes downward from left to right.The nature of a function can be analyzed by calculating...
290
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
8.4K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.8K
Decreased Body Temperature01:29

Decreased Body Temperature

A decreased body temperature can occur in patients with hypothermia and frostbite. Heat loss with extended cold exposure overpowers the body's ability to create heat, resulting in hypothermia. Core temperature readings help classify hypothermia. Mild hypothermia is temperatures between 32 °C (89.6 °F) and 35°C (95 °F) and is caused by impaired thermoregulation. Moderate hypothermia is temperatures between 28 C (82.4 °F) and 32 °C (89.6 °F) caused by...
1.1K