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Brucella-associated cervical bursitis in cattle
Auricelio Alves de Macedo1, Nayanna Ribeiro Galvão1, Joicy Cortez Sá2
1Universidade Estadual do Maranhão, São Luís, MA, Brazil.
Tropical Animal Health and Production
|November 11, 2018
Summary
Bovine brucellosis, a zoonotic disease causing economic losses, was investigated in cattle with cervical bursitis. Diagnostic tests confirmed Brucella spp. infection, highlighting an association between bursitis and the bacteria in Brazilian cattle herds.
Area of Science:
- Veterinary Medicine
- Infectious Diseases
- Bacteriology
Background:
- Bovine brucellosis presents significant risks to public health and the livestock industry.
- Cervical bursitis in cattle can be an indicator of underlying Brucella spp. infection.
- Understanding the prevalence and characteristics of Brucella in cattle is crucial for disease control.
Purpose of the Study:
- To diagnose Brucella spp. infection in cattle with cervical bursitis using multiple diagnostic methods.
- To investigate the association between cervical bursitis and Brucella spp. infection in cattle.
- To characterize the Brucella isolates found in the affected cattle population.
Main Methods:
- Serum samples from 47 cattle with bursitis were tested using the Rose Bengal Test (RBT) and 2-mercaptoethanol (2-ME) assay.
- Immunohistochemistry was performed on bursitis tissues to detect Brucella spp.
- Polymerase Chain Reaction (PCR) and bacterial isolation were used for bacterial detection and confirmation, followed by Multiplex AMOS-ENHANCED PCR for biovar characterization.
Main Results:
- The Rose Bengal Test (RBT) detected 85.1% positivity, with 78.7% confirmed by the 2-mercaptoethanol (2-ME) assay.
- Brucella spp. were detected in 34.0% of bursitis tissues via immunohistochemistry.
- PCR and bacterial isolation confirmed Brucella spp. in 63.8% of samples, with isolates identified as biovars 1, 2, and 4.
Conclusions:
- This study provides evidence for a significant association between cervical bursitis and Brucella spp. infection in cattle.
- Multiple diagnostic techniques are effective in identifying Brucella infection in cattle with clinical signs.
- The circulation of different Brucella biovars in bovine herds in Maranhão, Brazil, necessitates ongoing surveillance and control efforts.

