Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Lines01:16

Cell Lines

10.4K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
10.4K
Common Ion Effect03:24

Common Ion Effect

46.7K
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Châtelier’s principle. Consider the dissolution of silver iodide:
46.7K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

4.0K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
4.0K
Bone Cells and Tissue01:30

Bone Cells and Tissue

8.3K
Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
8.3K
Lossless Lines01:23

Lossless Lines

572
In electrical engineering, a lossless transmission line is characterized by a purely imaginary propagation constant and a resistive characteristic impedance. The ABCD parameters, which describe the relationship between the input and output voltages and currents, indicate an equivalent π circuit with an imaginary series impedance and a shunt admittance. This results in a transmission line that, when the product of the phase constant (beta) and the length of the line is less than pi, exhibits...
572
Common Respiratory Disorders01:31

Common Respiratory Disorders

1.4K
Respiratory disorders, a prevalent health concern globally, are generally divided into two primary categories: upper and lower respiratory tract disorders. The categorization is based on the area of the respiratory system they affect.
Upper respiratory disorders impact the airways above the vocal cords, encompassing areas like the nose, sinuses, and throat. Various conditions fall under this category, including the common cold and allergic rhinitis. These disorders can stem from several causes,...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond the Imodium, a One Health Discussion on Diarrhea and the Impact of Climate Change.

Medical science educator·2023
Same author

Sex and the basal mRNA synthesis machinery.

Wiley interdisciplinary reviews. RNA·2022
Same author

MicroRNA Profiles in Calcified and Healthy Aorta Differ: Therapeutic Impact of miR-145 and miR-378.

Physiological genomics·2020
Same author

Genetic background influences the impact of KLOTHO deficiency.

Physiological genomics·2020
Same author

Bone Morphogenetic Proteins (BMPs) and Bone Regeneration.

Methods in molecular biology (Clifton, N.J.)·2018
Same author

Unanswered Questions Regarding Sex and BMP/TGF-β Signaling.

Journal of developmental biology·2018

Related Experiment Video

Updated: Feb 2, 2026

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

8.0K

Common Cell Lines Used to Study Bone Morphogenetic Proteins (BMPs).

Jessica Ann Cottrell1, Donya Burgess2, Sarah Michaels2

  • 1Biological Sciences, Seton Hall University, South Orange, NJ, USA. Jessica.cottrell@shu.edu.

Methods in Molecular Biology (Clifton, N.J.)
|November 11, 2018
PubMed
Summary
This summary is machine-generated.

This chapter reviews methods for studying bone morphogenetic proteins (BMPs) in osteogenesis using immortalized mesenchymal cells. It details culture conditions and discusses reproducibility for reliable bone growth research.

Keywords:
BMP-2Mycoplasma

More Related Videos

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo
08:19

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

Published on: October 17, 2011

13.4K
Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors
11:49

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Published on: June 23, 2023

1.2K

Related Experiment Videos

Last Updated: Feb 2, 2026

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

8.0K
Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo
08:19

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

Published on: October 17, 2011

13.4K
Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors
11:49

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Published on: June 23, 2023

1.2K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Regenerative Medicine

Background:

  • Bone morphogenetic proteins (BMPs) are crucial for osteogenesis.
  • Understanding BMP functions requires robust experimental models.
  • Immortalized cell lines offer a reproducible system for studying osteogenic factors.

Purpose of the Study:

  • To review common immortalized mesenchymal cell types for BMP research.
  • To provide detailed protocols for cell culture and media conditions.
  • To highlight parameters for ensuring experimental reproducibility and cell line integrity.

Main Methods:

  • Literature review of established research methodologies.
  • Description of cell culture techniques for immortalized mesenchymal cells.
  • Discussion of quality control measures for experimental consistency.

Main Results:

  • Identification of suitable cell models for assessing osteogenic factors like BMP-2.
  • Provision of specific media formulations and culture parameters.
  • Emphasis on critical factors for reliable and reproducible experimental outcomes.

Conclusions:

  • Standardized methods using immortalized cells enhance the study of BMPs in osteogenesis.
  • Proper cell culture and authentication are vital for accurate results.
  • This chapter serves as a guide for researchers investigating bone formation.