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The antifibrinolytic activity of sulfamylon solution
J H Aldridge1, D J Weisdorf, J O Kucan
1Division of Plastic and Reconstructive Surgery, University of New Mexico school of Medicine, Albuqerque.
Abstract:
Sulfamylon (mafenide) solution, a potent experimental topical antimicrobial, is used in our burn unit to treat burn wounds both before and after skin grafting. The importance of fibrin to early graft adherence prompted this in vitro study of the effect of Sulfamylon upon fibrin clot. Assessing fibrinolysis by in vitro proteolysis of [125I] fibrin monomers, Sulfamylon, at relevant clinical concentrations, produced dose-related inhibition of streptokinase-mediated fibrinolysis. In addition, Sulfamylon had no intrinsic fibrinolytic activity. This antifibrinolytic property of Sulfamylon solution may, in vivo, protect against early graft loss when used on burn wounds and other potentially contaminated graft sites.
Insights
Sulfamylon (mafenide) solution inhibits fibrinolysis, preventing early clot breakdown. This antifibrinolytic effect may improve skin graft adherence in burn wound treatment.
Area of Science:
- Wound healing research
- Biochemistry
- Dermatology
Background:
- Sulfamylon (mafenide) solution is a topical antimicrobial used in burn units.
- Early adherence of skin grafts is crucial for successful wound healing.
- Fibrin plays a key role in the initial adherence of skin grafts.
Purpose of the Study:
- To investigate the in vitro effect of Sulfamylon solution on fibrin clot.
- To determine if Sulfamylon possesses antifibrinolytic properties.
Main Methods:
- In vitro study using proteolysis of radiolabeled [125I] fibrin monomers.
- Assessed fibrinolysis in the presence of varying concentrations of Sulfamylon.
- Evaluated the impact of Sulfamylon on streptokinase-mediated fibrinolysis.
Main Results:
- Sulfamylon demonstrated a dose-related inhibition of streptokinase-mediated fibrinolysis.
- Sulfamylon exhibited no intrinsic fibrinolytic activity.
- Relevant clinical concentrations of Sulfamylon were effective in inhibiting fibrinolysis.
Conclusions:
- Sulfamylon solution possesses antifibrinolytic properties.
- This antifibrinolytic action may protect against early graft loss in burn wound patients.
- Sulfamylon's use on burn wounds and contaminated graft sites could enhance graft adherence.