Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Digestive Functions of the Large Intestine01:20

Digestive Functions of the Large Intestine

2.2K
The large intestine is where the final stages of digestion happen. When the cecum receives chyme, it contains undigested carbohydrates that undergo fermentation. Gut bacteria ferment these carbohydrates to produce short-chain fatty acids that provide some energy and help synthesize essential vitamins.
As the chyme moves to the colon, it triggers two characteristic sluggish contractions - haustral churning and mass peristalsis. Haustral churning involves the rhythmic contraction and relaxation...
2.2K
Epithelial Tissues and Their Functions01:23

Epithelial Tissues and Their Functions

40.6K
Epithelial tissues are large sheets of cells covering all of the surfaces of the body. These surfaces can be internal or external, for example, skin, airways, the digestive tract, the urinary system, and the reproductive system. Hollow organs and body cavities that do not connect to the body's exterior, including blood vessels and serous membranes, are lined by epithelial tissue known as the endothelium.
Epithelial tissues provide the body's first line of protection from physical,...
40.6K
Anatomy of the Intestines01:23

Anatomy of the Intestines

87.4K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
87.4K
Stereotype Content Model02:16

Stereotype Content Model

15.4K
The Stereotype Content Model (SCM) was first proposed by Susan Fiske and her colleagues (Fiske, Cuddy, Glick & Xu, 2002; see also Fiske, 2012 and Fiske, 2017). The SCM specifies that when someone encounters a new group, they will stereotype them based on two metrics: warmth—or that group’s perceived intent, and how likely they are to provide help or inflict harm—and competence—or their ability to carry out that objective. Depending on the warmth-competence...
15.4K
Physiological Barriers01:25

Physiological Barriers

5.2K
Physiological barriers are semi-permeable cellular structures restricting drug diffusion into intracellular compartments and tissues. There are six types of physiological barriers: blood endothelial, cell membrane, blood-brain, blood-cerebrospinal fluid (CSF), blood-placenta, and blood-testis barriers.
The blood endothelial barrier is the most porous of these. It allows all small ionized, un-ionized, and lipophilic molecules to pass through the endothelial lining into the interstitial space...
5.2K
Small Intestine01:15

Small Intestine

3.9K
The small intestine is primarily responsible for digestion and nutrient absorption. It spans from the pyloric sphincter to the ileocecal valve and connects to the large intestine.
The small intestine is divided into three main sections - the duodenum, jejunum, and ileum. The duodenum, approximately 25 cm long, is nearest the stomach. It acts as a 'mixing bowl,' where chyme (partially digested food) blends with digestive enzymes from the pancreas and liver. The duodenum's unique...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing the reaction to and efficacy of the Screener drug discovery and development board game as a pedagogical tool in postgraduate courses.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2024
Same author

High-Fat Diet Induces Disruption of the Tight Junction-Mediated Paracellular Barrier in the Proximal Small Intestine Before the Onset of Type 2 Diabetes and Endotoxemia.

Digestive diseases and sciences·2020
Same author

A jaboticaba extract prevents prostatic damage associated with aging and high-fat diet intake.

Food & function·2020
Same author

Preclinical toxicity of innovative molecules: In vitro, in vivo and metabolism prediction.

Chemico-biological interactions·2019
Same author

International practices in the dietary management of fructose 1-6 biphosphatase deficiency.

Orphanet journal of rare diseases·2018
Same author

Acute and chronic exposure to high levels of glucose modulates tight junction-associated epithelial barrier function in a renal tubular cell line.

Life sciences·2017

Related Experiment Video

Updated: Feb 2, 2026

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice
10:28

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Published on: May 24, 2018

16.4K

Intestinal luminal content from high-fat-fed prediabetic mice changes epithelial barrier function in vitro.

R B Oliveira1, L P Canuto1, C B Collares-Buzato1

  • 1Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.

Life Sciences
|November 11, 2018
PubMed
Summary

High-fat diets alter gut contents, impairing intestinal barrier function and tight junctions. This disruption may contribute to obesity-related type 2 diabetes development.

Keywords:
Caco-2 cellsIntestinal paracellular barrierObesityTight junctionType 2 diabetes mellitus

More Related Videos

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function
09:40

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Published on: July 29, 2021

7.3K
Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
09:24

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model

Published on: February 11, 2021

8.0K

Related Experiment Videos

Last Updated: Feb 2, 2026

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice
10:28

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Published on: May 24, 2018

16.4K
Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function
09:40

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Published on: July 29, 2021

7.3K
Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
09:24

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model

Published on: February 11, 2021

8.0K

Area of Science:

  • Gastroenterology
  • Metabolic Diseases
  • Cell Biology

Background:

  • High-fat diets (HFD) alter the intestinal lumen environment.
  • Gut dysbiosis and increased intestinal permeability are implicated in type 2 diabetes mellitus (T2DM) pathogenesis.
  • Intestinal barrier disruption may trigger inflammation and insulin resistance.

Purpose of the Study:

  • To investigate the effect of intestinal luminal content from HFD-fed mice on epithelial barrier function.
  • To examine the impact on tight junction (TJ)-mediated epithelial barriers in Caco-2 and MDCK cells.

Main Methods:

  • Isolated small and large intestine luminal content from control and HFD-fed prediabetic mice.
  • Exposed Caco-2 and MDCK epithelial cell lines to luminal content.
  • Measured transepithelial electrical resistance (TEER) and paracellular flux.
  • Assessed TJ protein expression (claudins, occludin, ZO-1).

Main Results:

  • Small intestine luminal content from HFD-fed mice significantly decreased TEER and increased paracellular flux, with reduced TJ proteins.
  • Large intestine luminal content from HFD-fed mice increased paracellular flux and altered TJ protein distribution.
  • These changes indicate disruption of the TJ barrier integrity.

Conclusions:

  • Altered intestinal luminal components from HFD exposure impair TJ structure and function in vitro.
  • This supports a role for the intestinal paracellular barrier in obesity-related T2DM pathogenesis.