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Updated: Feb 2, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
Multi-fingerprint profiling combined with chemometric methods for investigating the quality of Astragalus
Chan-Yi Li1, Hong-Yuan Chen2, Wu-Ping Liu1
1The Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Abstract:
It is a challenge to ascertain the quality of polysaccharides due to their complex chemical structure; therefore, multi-fingerprint profiling was used to investigate the quality of Astragalus polysaccharides (APS) harvested from Inner Mongolia (NM) and Gansu (GS) with the help of chemometric analysis. Additionally, FT-IR and 1H NMR were applied to characterize the chemical structure of the harvested APS. The spectral fingerprinting results indicated that APS had reduced similarity when they were from different origins. Further, PCA showed that NM and GS could be distinguished and that the main differences from the loading plots were in the absorption intensity of carbonyls and H1 signals of Galp and β-glucose. Moreover, UPLC/Q-TOF-MS fingerprints were established based on the monosaccharide composition of the APS. The concentration of monosaccharides and results of cluster analysis indicated that GlcA might be an indicator that can be used to distinguish NM and GS. Overall, this multiple fingerprint method was stable, comprehensive and valid for monitoring APS quality.
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