Protocol for Agrobacterium-Mediated Transformation and Transgenic Plant Production of Switchgrass
1Department of Horticulture, Plant Biotechnology Resource and Outreach Center, Michigan State University, East Lansing, MI, USA. chenqiu1@msu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|November 12, 2018
Abstract:
Switchgrass (Panicum virgatum L.) is one of the most important bioenergy crops for lignocellulose ethanol production. Molecular breeding provides a powerful tool to supplement conventional switchgrass breeding by introducing or editing genes of interest. In this chapter, we describe Agrobacterium tumefaciens-mediated transformation protocols for lowland tetraploid switchgrass cultivar Alamo.
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