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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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What is Genetic Engineering?00:49

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Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
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CRISPR/Cas9 for Mutagenesis in Rice.

Si Nian Char1, Riqing Li1, Bing Yang2

  • 1Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 12, 2018
PubMed
Summary

This study presents a CRISPR/Cas9 genome editing protocol for rice. This method enables precise genetic modifications to enhance crop performance and understand gene functions.

Keywords:
Agrobacterium-mediated rice transformationCRISPR/Cas9Genome editingRiceTargeted mutagenesis

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Area of Science:

  • Agricultural biotechnology
  • Molecular biology
  • Plant genetics

Background:

  • CRISPR/Cas9 is a powerful genome editing tool widely used in eukaryotes.
  • This technology offers significant potential for improving crop plants, including rice, by enhancing traits like yield and stress resistance.
  • Developing specific protocols is crucial for effective application in different plant species.

Purpose of the Study:

  • To present a detailed CRISPR/Cas9-mediated genome editing protocol specifically for rice.
  • To facilitate precise genomic alterations in rice for research and crop improvement.
  • To provide a comprehensive guide for researchers working with rice genome editing.

Main Methods:

  • Detailed protocol for CRISPR/Cas9-mediated genome editing in rice.
  • Includes single-guide RNA design, vector construction, and plant transformation.
  • Covers mutant screening processes for identifying desired genetic modifications.

Main Results:

  • Establishment of a functional CRISPR/Cas9 genome editing protocol for rice.
  • Demonstration of precise genetic alterations in rice using the developed protocol.
  • Provides a repeatable methodology for rice genome editing.

Conclusions:

  • The presented CRISPR/Cas9 protocol is effective for rice genome editing.
  • This protocol can aid in understanding gene function and improving rice varieties.
  • Facilitates advancements in rice biotechnology and crop improvement strategies.