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Conditioned media from human umbilical cord blood-derived mesenchymal stem cells stimulate rejuvenation function in

Yoon-Jin Kim1, Dong Hee Seo1, Seung Hee Lee1

  • 1Stem Cells and Regenerative Bioengineering Institute, Kangstem Biotech Co., Ltd., 2nd floor, Biotechnology center, #81 Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.

Biochemistry and Biophysics Reports
|November 13, 2018
PubMed

Insights

Human umbilical cord blood-derived mesenchymal stem cells conditioned media (USC-CM) promote skin rejuvenation. USC-CM enhances fibroblast growth and collagen production, reducing wrinkles and increasing dermal density in women.

Area of Science:

  • Dermatology and Regenerative Medicine
  • Stem Cell Biology
  • Biochemistry

Background:

  • Skin aging is a significant concern, with rejuvenation strategies focusing on prevention.
  • Mesenchymal stem cells (MSCs) secrete cytokines crucial for wound healing and tissue repair.
  • Understanding MSC-derived factors for anti-aging treatments is an active research area.

Purpose of the Study:

  • To investigate the effects of human umbilical cord blood-derived mesenchymal stem cells conditioned media (USC-CM) on cutaneous wound healing and collagen synthesis.
  • To identify key rejuvenation factors within USC-CM and evaluate their specific roles.
  • To assess the efficacy of USC-CM in improving skin aging indicators.

Main Methods:

  • In vitro analysis of USC-CM composition, identifying growth factors like EGF, bFGF, PDGF, HGF, Collagen type 1, and GDF-11.
  • In vitro studies on Human Dermal Fibroblasts (HDFs) to assess USC-CM's impact on cell growth, migration, and extracellular matrix (ECM) production.
  • In vivo study involving topical application of USC-CM to women to evaluate anti-wrinkle effects and dermal density.

Main Results:

  • USC-CM contains potent skin rejuvenation factors, notably growth differentiation factor-11 (GDF-11).
  • In vitro, USC-CM significantly stimulated HDF growth and ECM production compared to other MSC-CM.
  • GDF-11 demonstrated acceleration of HDF growth, migration, and ECM production.
  • Topical USC-CM application in women resulted in anti-wrinkle effects and increased dermal density.

Conclusions:

  • USC-CM is a promising source of factors for skin rejuvenation.
  • The identified growth factors, particularly GDF-11, contribute to enhanced fibroblast activity and ECM synthesis.
  • USC-CM demonstrates clinical potential for mitigating skin aging through topical application.
Keywords:
Conditioned mediaCosmeticsMesenchymal stem cellsSkinUmbilical cord blood-derived mesenchymal stem cells conditioned media (USC-CM)

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