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Updated: Feb 2, 2026

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
Published on: October 25, 2016
High-throughput determination of the antigen specificities of T cell receptors in single cells
Shu-Qi Zhang1, Ke-Yue Ma2, Alexandra A Schonnesen3
1McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA.
Abstract:
We present tetramer-associated T-cell receptor sequencing (TetTCR-seq) to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcription and translation. We applied TetTCR-seq to identify patterns in TCR cross-reactivity with cancer neoantigens and to rapidly isolate neoantigen-specific TCRs with no cross-reactivity to the wild-type antigen.
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