Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

98.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.5K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

4.1K
4.1K
Sequences01:29

Sequences

277
Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
277
Sanger Sequencing01:57

Sanger Sequencing

774.5K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
774.5K
Arithmetic Sequences01:30

Arithmetic Sequences

239
An arithmetic sequence is a structured arrangement of numbers where each term is derived by adding a constant value, known as the common difference, to the previous term. This consistent pattern allows for the efficient computation of any term within the sequence as well as the cumulative sum of multiple terms. The formula for finding the nth term of an arithmetic sequence is:Here, aₙ represents the nth term of the sequence, a is the first term, d is the common difference, and n is the...
239

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole-genome sequences provide insights into the formation and adaptation of human populations in the Himalayas.

Current biology : CB·2025
Same author

Reply.

Ophthalmology·2025
Same author

Identifying a monozygotic twin brother as a donor of DNA in minimal, mixed forensic stains - A case example.

Forensic science international. Genetics·2025
Same author

Survival in Patients with Uveal Melanoma Is Linked to Genetic Variation at HERC2 Single Nucleotide Polymorphism rs12913832.

Ophthalmology·2024
Same author

Mitochondrial Transport from Mesenchymal Stromal Cells to Chondrocytes Increases DNA Content and Proteoglycan Deposition <i>In Vitro</i> in 3D Cultures.

Cartilage·2022
Same author

Large-scale pedigree analysis highlights rapidly mutating Y-chromosomal short tandem repeats for differentiating patrilineal relatives and predicting their degrees of consanguinity.

Human genetics·2022

Related Experiment Video

Updated: Feb 2, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.0K

From next generation sequencing to now generation sequencing in forensics.

Peter de Knijff1

  • 1Department of Human Genetics, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, the Netherlands.

Forensic Science International. Genetics
|November 13, 2018
PubMed
Summary

Massively parallel sequencing (MPS) offers significant advantages over capillary electrophoresis (CE) for forensic DNA analysis, particularly with mixed samples. Understanding MPS intricacies is crucial for its routine adoption in forensic science and court.

Keywords:
Massively parallel sequencing (MPS)Next generation seqeuncing (NGS)Short tandem repeat (STR)

More Related Videos

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
09:59

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Published on: October 11, 2021

3.8K
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.8K

Related Experiment Videos

Last Updated: Feb 2, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.0K
Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
09:59

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Published on: October 11, 2021

3.8K
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.8K

Area of Science:

  • Forensic genetics
  • Molecular biology
  • Genomics

Background:

  • Genetic diagnostic fields like Oncogenetics and Clinical Genetics have widely adopted Massively Parallel Sequencing (MPS) since 2010.
  • Forensic application of MPS for Short Tandem Repeats (STRs) is not yet a routine diagnostic tool, unlike its established use in clinical settings.
  • Capillary Electrophoresis (CE) based genotyping of STRs has been a forensic standard since the early 1990s.

Purpose of the Study:

  • To elucidate the fundamental differences between MPS and CE for STR genotyping in forensics.
  • To highlight essential knowledge for DNA experts when presenting MPS-based evidence in court.
  • To define necessary components for forensic investigation reports utilizing MPS technology.

Main Methods:

  • Comparative analysis of Massively Parallel Sequencing (MPS) and Capillary Electrophoresis (CE) for Short Tandem Repeat (STR) analysis.
  • Discussion of the complexities and nuances specific to MPS in a forensic context.
  • Review of requirements for courtroom testimony and forensic reporting of MPS-derived data.

Main Results:

  • MPS offers superior deconvolution capabilities for mixed-source DNA samples compared to CE.
  • MPS can reveal genetic information unattainable through traditional CE methods.
  • Significant differences exist in data interpretation and application between MPS and CE STR genotyping.

Conclusions:

  • Wider acceptance and routine use of MPS in forensic science are advocated due to its advanced capabilities.
  • Comprehensive understanding of MPS is necessary for its effective implementation and legal acceptance in forensic investigations.
  • MPS technology promises to enhance the resolution and scope of forensic DNA analysis.