Related Experiment Video

Updated: Feb 2, 2026

Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke
11:32

Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke

Published on: January 3, 2025

1.6K

MALAT1 Activates the P53 Signaling Pathway by Regulating MDM2 to Promote Ischemic Stroke

Ting Zhang1, Hongmei Wang2, Qiang Li3

  • 1Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China549832649@qq.com.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|November 13, 2018
PubMed
Abstract

Insights

This study investigated MALAT1 and MDM2's role in ischemic stroke via the p53 pathway. Targeting MALAT1/MDM2 reduced cell death and infarct size, suggesting a new therapeutic strategy.

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Genetics

Background:

  • Ischemic stroke is a leading cause of death and disability.
  • The p53 signaling pathway plays a critical role in cellular responses to stress.
  • Long non-coding RNAs (lncRNAs) and their involvement in stroke pathogenesis are increasingly recognized.

Purpose of the Study:

  • To evaluate the effect of MALAT1 and MDM2 on ischemic stroke.
  • To elucidate the regulatory role of MALAT1 and MDM2 in the p53 signaling pathway.
  • To explore the therapeutic potential of targeting the MALAT1/MDM2/p53 axis in ischemic stroke.

Main Methods:

  • Bioinformatics analysis to identify dysregulated lncRNAs, mRNAs, and pathways.
  • In vitro (Oxygen-glucose deprivation/reoxygenation) and in vivo (middle cerebral artery occlusion/reperfusion) models of ischemic stroke.
  • Quantitative real-time PCR (qRT-PCR), Western blot, fluorescence in situ hybridization (FISH), MTT assay, flow cytometry, and immunohistochemistry were employed.

Main Results:

  • MALAT1 and MDM2 were found to be upregulated in ischemic stroke tissues and models.
  • The MALAT1/MDM2/p53 signaling pathway was activated in response to ischemic conditions.
  • Downregulation of MALAT1 and MDM2 promoted cell proliferation, reduced apoptosis, and decreased infarct size in a mouse model of ischemic stroke.

Conclusions:

  • The MALAT1/MDM2/p53 signaling pathway axis is implicated in the pathogenesis of ischemic stroke.
  • Targeting MALAT1 and MDM2 demonstrates a neuroprotective effect, reducing stroke-induced brain damage.
  • This pathway represents a promising therapeutic target for clinical intervention in ischemic stroke patients.
Keywords:
Ischemic strokeMALAT1MDM2P53 pathway

More Related Videos

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

8.0K
Purification of Ubiquitinated p53 Proteins from Mammalian Cells10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.8K

Related Concept Videos

Regulation of Stroke Volume01:27

Regulation of Stroke Volume

The regulation of stroke volume, which is the amount of blood the heart pumps out during each heartbeat, is critical for maintaining a healthy circulatory system. Stroke volume is influenced by three main factors: preload, contractility, and afterload.
Preload refers to the degree of stretch on the heart before it contracts. It's analogous to the stretching of a rubber band; the more it's stretched, the more forcefully it snaps back. This concept is encapsulated in the Frank-Starling law of the...
5.2K
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.6K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.1K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.4K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.5K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.6K