Related Experiment Video

Updated: Feb 2, 2026

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis
08:25

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Published on: September 26, 2022

3.5K

Effect of 2, 6-Dichlorobenzonitrile on Amoebicidal Activity of Multipurpose Contact Lens Disinfecting Solutions

Eun-Kyung Moon1, Seungeun Lee2, Fu-Shi Quan1,3

  • 1Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, Korea.

The Korean Journal of Parasitology
|November 14, 2018
PubMed

Insights

Multipurpose contact lens disinfecting solutions (MPDS) show limited efficacy against Acanthamoeba cysts. Adding 2,6-dichlorobenzonitrile (DCB) enhanced activity against immature cysts, suggesting DCB as a potential adjuvant for MPDS.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Parasitology

Background:

  • Multipurpose contact lens disinfecting solutions (MPDS) are crucial for contact lens hygiene.
  • Current MPDS exhibit insufficient efficacy against Acanthamoeba cysts, a significant cause of microbial keratitis.
  • 2,6-dichlorobenzonitrile (DCB), a cellulose synthesis inhibitor, shows potential in inhibiting Acanthamoeba encystation.

Purpose of the Study:

  • To evaluate the potential of 2,6-dichlorobenzonitrile (DCB) as an agent to enhance the amoebicidal activity of MPDS against Acanthamoeba cysts.
  • To assess the efficacy of commercial MPDS, alone and in combination with DCB, against both mature and immature Acanthamoeba cysts.

Main Methods:

  • Eight commercial MPDS were tested for amoebicidal activity against Acanthamoeba cysts.
  • The efficacy of MPDS combined with 100 μM DCB was evaluated against mature and immature cysts.
  • Cytotoxicity of DCB and its mixtures with MPDS was assessed on human corneal epithelial cells.

Main Results:

  • All tested MPDS showed inadequate activity against mature Acanthamoeba cysts.
  • MPDS solutions e, f, and h demonstrated activity against immature cysts.
  • Combining MPDS with 100 μM DCB enhanced amoebicidal activity against immature cysts, particularly for solutions d, e, f, and h.
  • Solution d showed improved amoebicidal activity upon DCB addition.
  • No cytotoxicity was observed in human corneal epithelial cells treated with DCB or DCB-MPDS mixtures.

Conclusions:

  • DCB shows promise in enhancing the amoebicidal efficacy of MPDS against Acanthamoeba immature cysts.
  • DCB may serve as a valuable adjuvant to improve contact lens disinfection protocols.
  • Further research is warranted to explore DCB's role in combating Acanthamoeba infections related to contact lens wear.
Keywords:
AcanthamoebaDCBMPDSamoebicidal effectcytotoxicity

More Related Videos

Determining Viral Disinfection Efficacy of Hot Water Laundering06:57

Determining Viral Disinfection Efficacy of Hot Water Laundering

Published on: June 21, 2022

3.0K
Author Spotlight: Advancing Understanding of Age-Related Lens Stiffness Changes05:19

Author Spotlight: Advancing Understanding of Age-Related Lens Stiffness Changes

Published on: April 5, 2024

2.8K

Related Concept Videos

Cleaning, Sterilization, and Disinfection01:30

Cleaning, Sterilization, and Disinfection

Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
10.3K
Solution Concentration and Dilution02:59

Solution Concentration and Dilution

The relative amount of a given solution component is known as its concentration. Often, though not always, a solution contains one component with a concentration that is significantly greater than that of all other components. This component is called the solvent and may be viewed as the medium in which the other components are dispersed or dissolved. Solutions in which water is the solvent are, of course, very common on our planet. A solution in which water is the solvent is called an aqueous...
133.8K
Contact Angle01:13

Contact Angle

When a solid is dipped inside a liquid, the liquid surface becomes curved near the contact. For some solid–liquid interfaces, the liquid is pulled up along the solid, while for others, the liquid surface is convex or depressed near the solid surface. This phenomenon can be explained using the concept of cohesive and adhesive forces.
The adhesive force is the molecular force between molecules of different materials, that is, between the molecules of the solid and the liquid. The cohesive...
19.7K
Contact-dependent Signaling01:19

Contact-dependent Signaling

Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
47.0K
Weak Acid Solutions04:02

Weak Acid Solutions

Few compounds act as strong acids. A far greater number of compounds behave as weak acids and only partially react with water, leaving a large majority of dissolved molecules in their original form and generating a relatively small amount of hydronium ions. Weak acids are commonly encountered in nature, being the substances partly responsible for the tangy taste of citrus fruits, the stinging sensation of insect bites, and the unpleasant smells associated with body odor. A familiar example of a...
43.1K
Ideal Solutions02:24

Ideal Solutions

According to Raoult’s law, the partial vapor pressure of a solvent in a solution is equal or identical to the vapor pressure of the pure solvent multiplied by its mole fraction in the solution. However, Raoult's Law is only valid for ideal solutions. For a solution to be ideal, the solvent-solute interaction must be just as strong as a solvent-solvent or solute-solute interaction. This suggests that both the solute and the solvent would use the same amount of energy to escape to the...
22.4K