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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.8K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

8.9K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
8.9K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.5K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.5K
Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

4.3K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
4.3K
Mass Spectrometry of Amines01:15

Mass Spectrometry of Amines

5.4K
In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
5.4K
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

1.5K
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
1.5K

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Related Experiment Video

Updated: Feb 2, 2026

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
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Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications.

Edward E K Baidoo1,2, Veronica Teixeira Benites3,4

  • 1Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA. eebaidoo@lbl.gov.

Methods in Molecular Biology (Clifton, N.J.)
|November 14, 2018
PubMed
Summary

Understanding cellular metabolism is key, and metabolomics offers insights into microbial metabolic networks. Mass spectrometry is a vital tool for these analyses in environmental and disease research.

Keywords:
CE-MSData analysisGC-MSHuman diseaseLC-MSMass spectrometryMetabolic quenchingMetabolite extractionMetabolomicsMicrobialMicrobial communities

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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
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Area of Science:

  • Metabolomics and Microbiology

Background:

  • The metabolome closely reflects cellular metabolic activities and influences other 'omics' layers.
  • Understanding gene function increasingly relies on metabolomic data.

Purpose of the Study:

  • To detail the technical aspects of mass spectrometry-based metabolomics.
  • To discuss the application of these techniques in microbiological research.

Main Methods:

  • Mass spectrometry is the primary technique for metabolomic measurements.
  • Focus on the technical considerations of mass spectrometry for metabolomics.

Main Results:

  • Mass spectrometry-based metabolomics has significantly advanced environmental microbiology.
  • These methods are crucial for understanding human disease-related microbiology.

Conclusions:

  • Mass spectrometry is essential for exploring microbial metabolic networks.
  • This chapter provides a technical overview and application examples for microbiological research.