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In vivo 19F MRI for Cell Tracking
10:05

In vivo 19F MRI for Cell Tracking

Published on: November 25, 2013

15.4K

Preclinical 19F MRI cell tracking at 3 Tesla

Ashley V Makela1,2, Paula J Foster3,4

  • 1Robarts Research Institute, London, ON, Canada. amakela@uwo.ca.

Magma (New York, N.Y.)
|November 14, 2018
PubMed
Abstract

Insights

Fluorine-19 (19F) MRI enables cell tracking in tumors at 3 Tesla. This method can detect as few as 25,000 cells, offering a new imaging biomarker for tumor aggressiveness.

Area of Science:

  • Biomedical Imaging
  • Magnetic Resonance Imaging
  • Cellular Biology

Background:

  • 19F MRI offers advantages for cell tracking compared to iron-based methods.
  • Preclinical 19F cell tracking has been limited to high magnetic field strengths (>3 T).
  • 19F MRI can serve as an imaging biomarker for tumor aggressiveness by detecting macrophages.

Purpose of the Study:

  • To develop methods for 19F MRI cell tracking in mice using a 3 T clinical scanner.
  • To assess the feasibility of detecting and quantifying 19F-labeled macrophages in tumors at 3 T.

Main Methods:

  • Perfluorocarbon (PFC)-labeled macrophages were imaged at 3 T.
  • 19F spin quantification was validated using NMR spectroscopy.
  • In vivo 19F MRI was performed on tumor-bearing mice at 9.4 T and 3 T, with varying NEX at 3 T.

Main Results:

  • Detection of as few as 25,000 cells in cell pellets at 3 T.
  • 19F quantification in cell pellets by 3 T MRI correlated well with NMR spectroscopy.
  • 19F signal was consistently observed in the liver, spleen, and tumor in all mice at both 9.4 T and 3 T, with no significant difference in spin quantification.

Conclusions:

  • This study successfully demonstrates the detection and quantification of 19F signal in murine tumors using 19F MRI at 3 T.
  • 19F MRI at 3 T is a viable method for preclinical cell tracking, particularly for macrophage detection in tumors.
Keywords:
19-Fluorine (19F)CancerCell trackingMagnetic resonance imaging (MRI)Tumor-associated macrophage (TAM)

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