Related Experiment Video
Updated: Feb 2, 2026

Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
Published on: October 10, 2014
Analyzing the suitability of a baffled orbitally shaken bioreactor for cells cultivation using the computational
Likuan Zhu1,2, Boyan Song1, Zhenlong Wang1
1School of Mechatronics Engineering, Harbin Inst. of Technology, Harbin, Heilongjiang, 150001, China.
Abstract:
Orbitally shaken bioreactors (OSRs) is one of important bioreactors for mammalian cells cultivation in suspension, especially for the screening of valuable microorganisms and in basic bioprocess development experiments. However, the suitability of OSRs for cells culture in large scale is still under development. In this article, a new kind of OSRs with baffle structure was proposed and a three-dimensional CFD model was established to analyze the influence of baffle structure on the flow field. Lower installation height of baffles was found suitable for improving the mixing efficiency. Compared to the unbaffled OSR, the baffled OSR could enhance the level of oxygen transfer largely but the oxygen transfer rate was independent on the baffle installation height. Moreover, as the baffle installation height increased, the energy transferred for liquid motion was decreased. Finally, the shear stress of the baffled OSRs proposed was gentle for mammalian cells growth. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2746, 2019.
Related Concept Videos
Atomic Orbitals
Molecular Orbital Theory I
Molecular Orbital Theory II
Hybridization of Atomic Orbitals I
Hybridization of Atomic Orbitals II
The Energies of Atomic Orbitals

