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Related Experiment Videos

The relationship between macrophages and C5b-9 complement complexes in human atherosclerosis.

H G Rus1, F Niculescu, R Vlaicu

  • 1Medical Clinic No.1, Cluj-Napoca, Romania.

Clinical Immunology and Immunopathology
|September 1, 1988
PubMed
Summary

The terminal C5b-9 complement complex is found on macrophages in human atherosclerotic arteries. This suggests C5b-9 formation on these cells may drive inflammation and disease progression.

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Area of Science:

  • Immunology
  • Pathology
  • Cardiovascular Science

Background:

  • Atherosclerosis is a chronic inflammatory disease of arteries.
  • Macrophages play a key role in atherosclerotic lesion development.
  • The terminal complement complex (C5b-9) has been implicated in inflammatory conditions.

Purpose of the Study:

  • To investigate the relationship between macrophages and terminal C5b-9 complement complexes in human atherosclerotic arteries.
  • To determine the localization of C5b-9 deposits relative to macrophages and their remnants.

Main Methods:

  • Monoclonal antibodies
  • Indirect immunoperoxidase staining
  • Immunogold silver staining
  • Double-labeling immunohistochemistry

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Main Results:

  • Macrophages, both intact cells and cell remnants, were identified in atherosclerotic arteries.
  • C5b-9 deposits were found to partially colocalize with intact macrophages and macrophage-derived debris.
  • Evidence of C5b-9 formation on activated or dying macrophages was observed.

Conclusions:

  • The terminal C5b-9 complement complex is present on macrophages in human atherosclerosis.
  • Formation of C5b-9 on macrophages may promote inflammatory events.
  • C5b-9 deposition on macrophages could contribute to the progression of atherosclerotic lesions.