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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
Published on: September 29, 2017
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Preparation of an injectable modified chitosan-based hydrogel approaching for bone tissue engineering
Kanyarat Saekhor1, Wanvisa Udomsinprasert2, Sittisak Honsawek3
1Department of Materials Science, Faculty of Science, Chulalongkorn University, Phayathai, Pathumwan, Bangkok 10330, Thailand.
International Journal of Biological Macromolecules
|November 14, 2018
Summary
A novel injectable hydrogel was developed using carboxymethyl chitosan conjugated to alpha-cyclodextrin (CMCTSCD) and polyethylene glycol (PEG) for bone tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Polymer Chemistry
Background:
- Chitosan is a promising biomaterial for tissue engineering but requires modification for improved solubility and injectability.
- Developing injectable hydrogels that form under physiological conditions is crucial for bone regeneration therapies.
Purpose of the Study:
- To design and synthesize an injectable, self-gelling chitosan-based hydrogel system for bone tissue engineering.
- To enhance the properties of chitosan through carboxymethylation and alpha-cyclodextrin conjugation.
Main Methods:
- Chitosan was modified to carboxymethyl chitosan (CMCTS) and then conjugated with alpha-cyclodextrin (α-CD) to form CMCTSCD.
- CMCTSCD was crosslinked with poly(ethylene glycol) (PEG) via inclusion complex formation.
- The sol-to-gel transition time and hydrogel microstructure were characterized.
Main Results:
- The synthesized CMCTSCD/PEG hydrogel demonstrated improved water solubility and thixotropic properties.
- An optimal mole ratio of CMCTSCD to PEG (2:4) and a threading number of approximately 2.5 were determined.
- The hydrogel exhibited a sol-to-gel transition within 450 ± 10 minutes and possessed an interconnected porous structure.
Conclusions:
- The CMCTSCD/PEG hydrogel represents a novel injectable biomaterial suitable for bone tissue engineering.
- The developed hydrogel system offers potential for minimally invasive bone defect repair and regeneration.
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