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Updated: Feb 2, 2026
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Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells
Published on: May 11, 2020
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Microarray Profiling of TGF-β1-Induced Long Non-Coding RNA Expression Patterns in Human Lung Bronchial Epithelial
Wentao Hu1,2, Weiwei Pei1,2, Lin Zhu1,2
1School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China.
Summary
Long non-coding RNAs (lncRNAs) regulated by TGF-β1 are implicated in radiation-induced bystander effects and tumorigenesis. These lncRNAs offer new insights into cancer development following radiation exposure.
Area of Science:
- Molecular Biology
- Cancer Research
- Radiation Biology
Background:
- Transforming growth factor-beta 1 (TGF-β1) mediates radiation-induced bystander effects (RIBE), contributing to malignant transformation and tumorigenesis.
- The precise molecular mechanisms underlying TGF-β1's role in RIBE and subsequent tumorigenesis remain incompletely understood.
Purpose of the Study:
- To identify novel regulatory molecules involved in TGF-β1-mediated RIBE.
- To elucidate the functional roles of differentially expressed long non-coding RNAs (lncRNAs) in this process.
Main Methods:
- lncRNA microarray profiling of human lung bronchial epithelial cells (BEAS-2B) treated with TGF-β1.
- Gene Ontology (GO) and KEGG pathway analyses to predict functions of differentially expressed lncRNAs.
- Quantitative real-time PCR (qRT-PCR) and CCK-8 assays for validation and functional assessment.
Main Results:
- A total of 224 dysregulated lncRNAs were identified, with 6 validated by qRT-PCR.
- Pathway analyses indicated correlations between dysregulated lncRNAs and cell proliferation, transformation, and migration.
- lnc-THEMIS-2 and lnc-ITGB6-4 were found to mitigate TGF-β1-induced decreases in cell viability.
Conclusions:
- TGF-β1-induced lncRNAs play significant roles in oncogenic transformation and tumorigenesis.
- These findings enhance the understanding of mechanisms linking low-dose/low-dose-rate radiation to tumorigenesis via RIBE.
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