Related Experiment Video
Updated: Feb 2, 2026

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
Published on: November 16, 2015
Efficient immunoaffinity chromatography of lymphocytes directly from whole blood
Fabian Mohr1, Sabine Przibilla2, Franziska Leonhardt3
1Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universität München (TUM), Munich, Germany.
Abstract:
We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent functionality of purified cells.
Related Concept Videos
Affinity Chromatography
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Ion-Exchange Chromatography
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Production Efficiency
Trophic Efficiency

