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Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
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Multifunctional self-assembled monolayers via microcontact printing and degas-driven flow guided patterning.

Sang Hun Lee1, Won-Yeop Rho2, Seon Joo Park3

  • 1School of Chemical & Biological Engineering, Seoul National University, Seoul, 00826, Republic of Korea.

Scientific Reports
|November 15, 2018
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Summary

This study introduces a streamlined micropatterning technique combining microcontact printing (µCP) and microfluidic vacuum-assisted degas-driven flow guided patterning (DFGP). This facile method creates chemically defined surface architectures for biomolecules and nanoparticles without multiple steps.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Surface Chemistry

Background:

  • Soft lithography enables biological and chemical investigations.
  • Existing micropatterning methods involve multiple complex steps like layer-by-layer patterning and stamp alignment.

Purpose of the Study:

  • To develop a facile micropatterning method for creating chemically well-defined surface architectures.
  • To demonstrate a combined microcontact printing (µCP) and microfluidic vacuum-assisted degas-driven flow guided patterning (DFGP) approach.

Main Methods:

  • Utilized a poly(dimethylsiloxane) (PDMS) stamp integrating µCP and DFGP techniques.
  • Fabricated bi-composite micropatterned surfaces using functional molecular inks.

Main Results:

  • Successfully created patterns of fluorescein isothiocyanate labelled bovine serum albumin (FITC-BSA) and polyethylene glycol (PEG)-silane for biomolecule arrays.
  • Developed patterns of 3-aminopropyltriethoxysilane (APTES) and PEG-silane for self-assembled colloid gold nanoparticle monolayers.
  • Achieved bi-composite surface patterns without supplementary processing by optimizing molecular ink composition.

Conclusions:

  • The combined µCP-DFGP approach offers a simplified method for micropatterning.
  • This technique is highly applicable for fabricating microarrays of biomolecules and nanoparticle monolayers.