Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

65.1K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
65.1K
Real Number System01:27

Real Number System

1.1K
The real number system includes all numbers used for counting, measuring, and comparing quantities. Natural numbers are the basic counting numbers: 1, 2, 3, and so on. Integers expand this set by including zero and negative whole numbers: ..., –3, –2, –1, 0, 1, 2, .... Rational numbers are those that can be expressed as the ratio of two integers m/n, where n≠0. This includes fractions like 1/2​, integers such as 46, and decimals that terminate, such as 0.17, or...
1.1K
Self-Presentation: Self-Monitoring and Self-Handicapping02:05

Self-Presentation: Self-Monitoring and Self-Handicapping

44.8K
People can go to great lengths to protect their self-image and present themselves in ways that they want others to see them. Sociologist Erving Goffman presented the idea that a person is like an actor on a stage. Calling his theory dramaturgy, Goffman believed that we use “impression management” to present ourselves to others as we hope to be perceived. Each situation is a new scene, and individuals perform different roles depending on who is present (Goffman, 1959). Think about...
44.8K
Real Number Operations01:27

Real Number Operations

321
The concept of real numbers includes all the values that can be represented on a continuous number line. The system began with basic counting values used for enumeration. It later expanded to include values that represent the absence of quantity and opposites of the counting values. When situations required expressing parts of a whole or dividing quantities evenly, values capable of representing such proportions were developed. When written using decimal notation, these values can end or repeat...
321
Tissues01:18

Tissues

85.3K
Cells with similar structure and function are grouped into tissues. A group of tissues with a specialized function is called an organ. There are four main types of tissue in vertebrates: epithelial, connective, muscle, and nervous.
85.3K
Holter Monitor: 24-Hour Monitoring01:23

Holter Monitor: 24-Hour Monitoring

2.5K
Holter monitoring is a continuous electrocardiography (ECG) recording that tracks the heart's electrical activity over an extended period, generally 24 to 48 hours. This noninvasive diagnostic tool detects irregular heart rhythms that may not be captured during a standard ECG performed in a clinical setting.DeviceThe Holter monitor is a portable, small device connected to several electrodes on the patient's chest. These electrodes detect the heart's electrical signals and transmit them to the...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isothermal amplification techniques for rapid bacterial detection: alternatives to culturing and PCR-based methods.

Analytical methods : advancing methods and applications·2026
Same author

An Innovative In Vivo Model for CAR-T-Cell Therapy Development: Efficacy Evaluation of CD19-Targeting CAR-T Cells on Human Lymphoma, Using the Chicken CAM Assay.

International journal of molecular sciences·2026
Same author

MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis.

Journal of neuroinflammation·2024
Same author

IL23R-Specific CAR Tregs for the Treatment of Crohn's Disease.

Journal of Crohn's & colitis·2024
Same author

Development and Characterisation of a Whole Hybrid Sol-Gel Optofluidic Platform for Biosensing Applications.

Nanomaterials (Basel, Switzerland)·2022
Same author

Preclinical assessment of antigen-specific chimeric antigen receptor regulatory T cells for use in solid organ transplantation.

Gene therapy·2022

Related Experiment Video

Updated: Feb 2, 2026

Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies
07:12

Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies

Published on: November 19, 2020

2.6K

Optical Sensor for Real-Time pH Monitoring in Human Tissue.

Dorota Wencel1, Alicja Kaworek2, Tobias Abel3

  • 1School of Physical Science, Biomedical Diagnostic Institute, Dublin City University (DCU), Dublin 9, Ireland.

Small (Weinheim an Der Bergstrasse, Germany)
|November 15, 2018
PubMed
Summary
This summary is machine-generated.

A new fiber-based optical pH sensor enables real-time, in vivo monitoring of human tissue pH. This advanced sensor demonstrates high sensitivity and stability for continuous physiological measurements.

Keywords:
optical fibersratiometric optical pH sensorsreal-time in vivo pH monitoringsol-gel-based materials

More Related Videos

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
06:55

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Published on: October 19, 2021

4.4K
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
09:21

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Published on: January 4, 2016

10.4K

Related Experiment Videos

Last Updated: Feb 2, 2026

Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies
07:12

Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies

Published on: November 19, 2020

2.6K
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
06:55

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Published on: October 19, 2021

4.4K
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
09:21

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Published on: January 4, 2016

10.4K

Area of Science:

  • Biomedical Engineering
  • Optical Sensors
  • Materials Science

Background:

  • Accurate in vivo pH monitoring is crucial for understanding physiological processes and diagnosing diseases.
  • Existing pH monitoring technologies often face limitations in real-time, continuous, and minimally invasive applications.
  • Optical sensors offer potential advantages for in vivo measurements due to their non-ionizing nature and potential for miniaturization.

Purpose of the Study:

  • To develop and evaluate a novel fiber-based ratiometric optical pH sensor for real-time, continuous in vivo pH monitoring in human tissue.
  • To assess the sensor's performance characteristics, including sensitivity, resolution, stability, and response time, in both laboratory and in vivo settings.
  • To demonstrate the feasibility of the sensor for clinical applications through human evaluations.

Main Methods:

  • Fabrication of a fiber-based ratiometric optical pH sensor using a stable hybrid sol-gel sensing material deposited on a flexible plastic optical fiber tip.
  • Integration of the sensor with excitation and detection electronics for optical measurements.
  • Extensive laboratory testing, in vitro whole blood sample analysis, and in vivo human subject evaluation.

Main Results:

  • The developed pH sensor significantly outperforms the state-of-the-art in laboratory tests, exhibiting high sensitivity within the physiological pH range.
  • Achieved a resolution of 0.0013 pH units, excellent sensor-to-sensor reproducibility, long-term stability (0.003 pH units drift per 22 h), and a rapid response time (<2 min).
  • Demonstrated promising performance in in vitro whole blood samples and successful short-term in vivo deployment in human subjects.

Conclusions:

  • The fiber-based ratiometric optical pH sensor is a highly sensitive, stable, and reproducible device suitable for real-time, continuous in vivo pH monitoring.
  • The sensor's performance characteristics suggest its potential as a valuable tool for clinical diagnostics and physiological research.
  • Successful human evaluations confirm the sensor's viability for short-term in vivo applications in human tissue.