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The J-domain cochaperone Rsp1 interacts with Mto1 to organize noncentrosomal microtubule assembly

Juan Shen1,2,3, Tianpeng Li1,2,3, Xiaojia Niu1,2,3

  • 1Division of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.

Molecular Biology of the Cell
|November 15, 2018
PubMed

Insights

The J-domain cochaperone Rsp1 regulates Mto1 localization for microtubule nucleation at non-spindle pole body sites in fission yeast. Rsp1 absence enriches Mto1 at the SPB and nuclear envelope, while overexpression impairs all microtubule organization.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cytoskeleton Dynamics

Background:

  • Microtubule biogenesis is crucial for cellular processes and occurs at diverse intracellular sites.
  • In fission yeast (Schizosaccharomyces pombe), microtubule nucleation depends on Mto1 activity at the spindle pole body (SPB), nuclear envelope, and existing microtubules.
  • Regulation of Mto1 distribution to these nucleation sites remains largely unknown.

Purpose of the Study:

  • To investigate the regulatory mechanism controlling Mto1 localization for microtubule nucleation.
  • To identify factors specifying Mto1 distribution to non-SPB nucleation sites.

Main Methods:

  • Genetic analysis in Schizosaccharomyces pombe.
  • Co-immunoprecipitation to study protein interactions.
  • Fluorescence microscopy to visualize protein localization.

Main Results:

  • The J-domain cochaperone Rsp1 interacts with Mto1.
  • Rsp1 specifies Mto1 localization to non-SPB nucleation sites.
  • Absence of Rsp1 leads to Mto1 enrichment at the SPB and nuclear envelope.
  • Rsp1 overexpression disrupts Mto1 localization at all microtubule organization sites.

Conclusions:

  • Rsp1-Mto1 interaction is essential for orchestrating non-SPB microtubule formation.
  • Rsp1 acts as a key regulator of Mto1 localization, controlling microtubule nucleation.
  • This study reveals a novel mechanism for spatial regulation of microtubule organization.

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