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Biophysical Characterization of Flagellar Motor Functions
Published on: January 18, 2017
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The roles of a flagellar HSP40 ensuring rhythmic beating
Xiaoyan Zhu1, Emiliya Poghosyan2, Lenka Rezabkova2
1Department of Biological Sciences, Marquette University, Milwaukee, WI 53233.
Molecular Biology of the Cell
|November 15, 2018
Summary
Heat shock protein 40 (HSP40) acts as a structural chaperone in flagellar radial spokes, ensuring proper assembly and function for rhythmic beating. This study reveals HSP40
Area of Science:
- Cell Biology
- Molecular Biology
- Biophysics
Background:
- Heat shock protein 40s (HSP40s) typically function as cochaperones for HSP70s.
- Some HSP40s exhibit noncanonical roles beyond protein refolding.
- The radial spoke (RS) complex in flagella is crucial for motility.
Purpose of the Study:
- To investigate the noncanonical functions of HSP40 within the flagellar radial spoke complex.
- To elucidate the mechanism by which HSP40 influences RS structure and flagellar motility.
Main Methods:
- Gene disruption using MRC1 transposon in Chlamydomonas.
- Electron microscopy, cryo-electron tomography, and sub-tomogram analysis.
- Biochemical analysis and computational modeling.
Main Results:
- HSP40 disruption caused jerky flagella and altered RS morphologies.
- HSP40 stabilizes the RS, enabling interaction with the central pair apparatus for coordinated beating.
- HSP40 facilitates intraflagellar transport and assembly of RS precursors.
Conclusions:
- HSP40 acts as a structural chaperone for RS assembly in flagella.
- HSP40 plays a dual role in chaperoning and structural maintenance of the RS complex.
- This reveals novel insights into HSP40 versatility and flagellar biology.
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