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LncRNA GAS8-AS1 suppresses papillary thyroid carcinoma cell growth through the miR-135b-5p/CCND2 axis

NingHeng Chen1, DeTao Yin2, Bing Lun3

  • 1General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.

Bioscience Reports
|November 16, 2018
PubMed

Insights

GAS8 antisense RNA 1 (GAS8-AS1) suppresses papillary thyroid carcinoma (PTC) cell growth by targeting miR-135b-5p and regulating CCND2. This finding offers a potential therapeutic target for PTC treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Papillary thyroid carcinoma (PTC) is a common endocrine malignancy.
  • The role of long non-coding RNAs, such as GAS8 antisense RNA 1 (GAS8-AS1), in PTC development is not fully understood.

Purpose of the Study:

  • To investigate the function of GAS8-AS1 in PTC.
  • To elucidate the molecular mechanism underlying GAS8-AS1's role in PTC, focusing on its interaction with miR-135b-5p and CCND2.

Main Methods:

  • Quantitative reverse transcription PCR (QRT-PCR) and Western blot to assess gene and protein expression.
  • Cell Counting Kit-8 (CCK-8) assay and flow cytometry to evaluate cell proliferation and cell cycle.
  • Transfection of PTC cells with plasmids and small interfering RNAs (siRNAs).
  • In vivo tumor formation assays.

Main Results:

  • GAS8-AS1 expression was found to be downregulated in PTC cell lines.
  • Overexpression of GAS8-AS1 inhibited PTC cell proliferation and cell cycle progression.
  • GAS8-AS1 was identified as a direct target of miR-135b-5p.
  • GAS8-AS1 regulated CCND2 expression in an miR-135b-5p-dependent manner.
  • GAS8-AS1 suppressed tumor formation in vivo.

Conclusions:

  • GAS8-AS1 acts as a tumor suppressor in PTC.
  • GAS8-AS1 exerts its tumor-suppressive effects by inhibiting cell proliferation and cycle progression.
  • The GAS8-AS1/miR-135b-5p/CCND2 axis represents a novel regulatory pathway in PTC, suggesting potential therapeutic strategies.
Keywords:
CCND2GAS8-AS1PTCmiR-135b-5p

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