[A Screening Analysis Method for Stevia Sweetener in Processed Foods by LC-MS/MS]
Hiroyuki Miyakawa1, Mikio Sugiki1, Shoichi Tahara1
1Tokyo Metropolitan Institute of Public Health.
Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
|November 16, 2018
Summary
A new LC-MS/MS method accurately detects stevia sweeteners like stevioside (SS) and rebaudioside A (RS) in processed foods. This analysis confirmed the presence of these sweeteners in most tested food products.
Area of Science:
- Food Science
- Analytical Chemistry
- Biochemistry
Background:
- Stevia sweeteners are increasingly used in processed foods.
- Accurate quantification methods are essential for quality control and regulatory compliance.
- Existing methods may lack sensitivity or specificity for complex food matrices.
Purpose of the Study:
- To develop and validate a reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detecting stevia sweeteners in processed foods.
- To assess the prevalence of different stevia sweeteners (stevioside, rebaudioside A, and glycosyltransferase-treated stevia) in commercially available food products.
Main Methods:
- Extraction of stevia sweeteners from processed food samples using dialysis.
- Dilution of the dialysate with water.
- Analysis of stevia sweeteners by LC-MS/MS.
Main Results:
- Excellent recovery rates were achieved for stevioside (SS), rebaudioside A (RS), and glycosyltransferase-treated stevia (Gts) in spiked food samples.
- No interfering peaks were observed during the analysis.
- Out of 36 commercial products labeled as containing "stevia", 33 contained SS, 33 contained RS, and 11 contained Gts.
- Five products were found to contain both stevia extract and Gts.
Conclusions:
- The developed LC-MS/MS method is effective and accurate for screening stevia sweeteners in processed foods.
- The method demonstrates high recovery and specificity, making it suitable for routine analysis.
- The study highlights the widespread use of various forms of stevia sweeteners in the food industry.
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