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Related Concept Videos

Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

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Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
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Correlations02:20

Correlations

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Correlation means that there is a relationship between two or more variables (such as ice cream consumption and crime), but this relationship does not necessarily imply cause and effect. When two variables are correlated, it simply means that as one variable changes, so does the other. We can measure correlation by calculating a statistic known as a correlation coefficient. A correlation coefficient is a number from -1 to +1 that indicates the strength and direction of the relationship between...
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Correlation and Causation01:27

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Statistical tests can calculate whether there is a relationship, or correlation, between independent and dependent variables. An indirect relationship of the variables signifies a correlation, while a direct relationship shows causation. If it is determined that no connection exists between the variables, then the correlation is a coincidence.
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Asthma-I: Introduction01:29

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Asthma is a chronic respiratory ailment that requires careful management due to its varying symptoms and influencing factors. It is characterized by airway inflammation, bronchial hyperresponsiveness, and reversible airflow obstruction, leading to symptoms like wheezing, shortness of breath, chest tightness, and coughing. The symptom frequency and intensity may vary considerably over time. It is also linked to immune system responses to allergens and irritants, highlighting the complex...
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Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture01:26

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Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
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Asthma-III: Symptoms and Complications01:24

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Asthma, a common chronic respiratory condition, is classified considering the frequency and severity of symptoms alongside lung function impairment. Understanding this classification is essential for appropriate treatment and management. Here's a detailed look at the classification of asthma and its clinical features and complications:
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Bronchial Thermoplasty: A Novel Therapeutic Approach to Severe Asthma
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Sputum interleukin-25 correlates with asthma severity: a preliminary study.

Magdalena Paplińska-Goryca1, Elżbieta M Grabczak1, Marta Dąbrowska1

  • 1Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, Warsaw, Poland.

Postepy Dermatologii I Alergologii
|November 16, 2018
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Interleukin 25 (IL-25) is elevated in severe asthma and linked to neutrophilic inflammation, suggesting a key role in severe allergic airway disease.

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asthmaeosinophilsinduced sputuminterleukin 25neutrophils

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Area of Science:

  • Immunology
  • Respiratory Medicine
  • Allergy

Background:

  • Interleukin 25 (IL-25) is an epithelial cytokine implicated in allergic Th2 inflammation.
  • The specific role of IL-25 in diverse asthma phenotypes and its correlation with disease severity remain underexplored.

Purpose of the Study:

  • To investigate and contrast the messenger RNA (mRNA) and protein expression levels of IL-25.
  • To compare IL-25 expression in patients with mild-to-moderate asthma, severe asthma, and cough variant asthma (CVA).

Main Methods:

  • Quantitative analysis of IL-25 mRNA in induced sputum (IS) cells via real-time PCR.
  • Measurement of IL-25 protein concentration in IS supernatants using ELISA.
  • Study included 38 asthma patients (mild-to-moderate, severe, CVA) and 14 controls.

Main Results:

  • No significant difference in IL-25 mRNA or protein was found between controls and the overall asthma cohort.
  • Severe asthma patients exhibited lower IL-25 mRNA but higher IL-25 protein levels in sputum compared to controls and CVA.
  • Elevated IL-25 levels correlated with increased neutrophil and decreased eosinophil percentages in asthmatic airways.

Conclusions:

  • IL-25 is significantly associated with severe asthma phenotypes.
  • The findings suggest a pleiotropic role for IL-25 in asthma, particularly in neutrophilic airway inflammation.