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VEGI174 protein and its functional domain peptides exert antitumour effects on renal cell carcinoma

Qiang Zhao1, Baoan Hong2, Tiezhu Liu3

  • 1Department of Urology, Beijing Institute for Cancer Research, Beijing Cancer Hospital, Beijing 100142, P.R. China.

International Journal of Oncology
|November 16, 2018
PubMed

Insights

Vascular endothelial growth inhibitor (VEGI) protein and its peptides V7 and V8 show potential in treating renal cell carcinoma (RCC). These agents effectively inhibited cancer cell growth and tumor development in preclinical models with good tolerability.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Vascular endothelial growth inhibitor (VEGI) is an anti-angiogenic cytokine.
  • The therapeutic potential of VEGI174 protein and its functional domain peptides (V7, V8) in renal cell carcinoma (RCC) is unexplored.

Purpose of the Study:

  • To investigate the anti-cancer effects of biosynthesized VEGI174 protein, V7, and V8 peptides on RCC cell lines and a mouse xenograft model.
  • To evaluate the impact of these agents on cancer cell proliferation, migration, and invasion, as well as tumor growth in vivo.

Main Methods:

  • Treatment of A498 and 786-O RCC cell lines and a mouse xenograft model with VEGI174, V7, or V8.
  • Assessment of cellular functions (proliferation, migration, invasion) using the xCELLigence system.
  • Monitoring of tumor growth and mouse behavior (mobility, appetite, body weight) in xenograft models.

Main Results:

  • VEGI174, V7, and V8 significantly inhibited proliferation, migration, and invasion of RCC cell lines in a dose- and time-dependent manner.
  • All tested agents demonstrated significant tumor growth inhibition (TGI) in both A498 and 786-O xenograft models, with VEGI174 showing the highest TGI in A498.
  • No significant adverse effects on mouse body weight or mortality were observed, indicating good in vivo tolerability.

Conclusions:

  • VEGI174 protein and its functional peptides V7 and V8 possess significant anti-tumor properties against renal cell carcinoma.
  • These agents were well-tolerated in preclinical studies, suggesting their potential as novel therapeutic candidates for RCC treatment.
  • V7 and V8 peptides, derived from VEGI174, warrant further investigation for future clinical application in RCC therapy.
Keywords:
renal cell carcinomavascular endothelial growth inhibitorpeptidein vitroin vivo

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