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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

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CagA increases DNA methylation and decreases PTEN expression in human gastric cancer

Baogui Zhang1, Xiaobei Zhang2, Meng Jin1

  • 1Department of Gastrointestinal Surgery, Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, P.R. China.

Molecular Medicine Reports
|November 16, 2018
PubMed

Insights

Cytotoxin-associated gene A (CagA) in gastric cancer reduces tumor suppressor genes PTEN and Tet1. CagA also increases DNA methylation, leading to decreased PTEN, but Tet1 overexpression can reverse this effect.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Gastric cancer is a leading cause of cancer mortality globally.
  • Cytotoxin-associated gene A (CagA) is linked to gastric diseases.
  • PTEN and Tet1 are crucial tumor suppressor genes.

Purpose of the Study:

  • Investigate CagA's function in human gastric cancer.
  • Explore the relationship between CagA, PTEN, and Tet1 in gastric cancer.

Main Methods:

  • Constructed CagA overexpression and Tet1 interference lentiviral plasmids.
  • Utilized qPCR and Western blotting for gene and protein expression analysis.
  • Assessed PTEN methylation status using methylation-specific PCR.

Main Results:

  • CagA overexpression decreased PTEN, Tet1, and APOBEC3 gene/protein expression in HGC-27 cells.
  • Tet1 interference reduced PTEN expression, associated with increased DNA methylation.
  • CagA+ gastric cancer tissues showed decreased PTEN and Tet1 expression compared to non-cancerous tissues.

Conclusions:

  • CagA overexpression significantly downregulates PTEN, Tet1, and APOBEC3 family members in gastric cancer.
  • CagA promotes DNA methylation and reduces PTEN expression, an effect reversible by Tet1.
  • Findings may inform future therapeutic strategies for gastric cancer.
Keywords:
CagAgastric cancerTet1PTENmethylation

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