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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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When a solid is dipped inside a liquid, the liquid surface becomes curved near the contact. For some solid–liquid interfaces, the liquid is pulled up along the solid, while for others, the liquid surface is convex or depressed near the solid surface. This phenomenon can be explained using the concept of cohesive and adhesive forces.
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Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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The Fast Fourier Transform (FFT) is a computational algorithm designed to compute the Discrete Fourier Transform (DFT) efficiently. By breaking down the calculations into smaller, manageable sections, the FFT significantly reduces the computational complexity involved. Direct computation of an N-point DFT requires N2 complex multiplications, whereas the FFT algorithm needs only (N/2)log⁡2N multiplications, offering a much faster performance.
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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
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Fast 3D Microscopy Imaging of Contacts Between Surfaces Using a Fluorescent Liquid.

Dina Petrova1, Bart Weber2, Clémence Allain3

  • 1van 't Hoff Institute for Molecular Sciences , University of Amsterdam , Amsterdam P.O. Box 94157, 1090 GD , The Netherlands.

ACS Applied Materials & Interfaces
|November 16, 2018
PubMed
Summary

A new fluorescence microscopy technique rapidly visualizes 3D surface contact. It accurately measures nanoscale distances in seconds, advancing contact mechanics and tribology research.

Keywords:
3D imagingfluorescent probesinterfacesmechanical properties

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Area of Science:

  • Surface science
  • Microscopy
  • Nanotechnology

Background:

  • Understanding surface interactions is crucial for predicting material behavior.
  • Current methods for visualizing nanoscale contact are often time-consuming or lack 3D resolution.

Purpose of the Study:

  • To develop a rapid, direct 3D visualization method for surface contact.
  • To achieve subnanometer accuracy in distance measurements at the nanoscale.

Main Methods:

  • Utilizing fluorescence microscopy with a specialized fluorescent liquid.
  • Direct 3D imaging of the gap between two contacting surfaces.

Main Results:

  • Achieved rapid (seconds) 3D visualization of surface contact.
  • Determined distances up to several hundred nanometers with subnanometer accuracy.
  • Demonstrated the method's potential for real-time analysis.

Conclusions:

  • The novel method offers unprecedented capabilities for studying surface phenomena.
  • Opens new avenues for research in contact mechanics, friction, wear, and lubrication.
  • Provides a powerful tool for nanoscale surface metrology.