Related Experiment Video
Updated: Feb 2, 2026

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
Published on: April 25, 2022
In vivo cell type-specific CRISPR gene editing for sleep research
Hiroshi Yamaguchi1, Luis de Lecea1
1Department of Psychiatry and Behavioral Sciences, Stanford University, 1201 Welch Road, Stanford, CA, 94305, USA.
Abstract:
Sleep is an innate behavior conserved in all animals and, in vertebrates, is regulated by neuronal circuits in the brain. The conventional techniques of forward and reverse genetics have enabled researchers to investigate the molecular mechanisms that regulate sleep and arousal. However, functional interrogation of genes in specific cell subtypes in the brain remains a challenge. Here, we review the background of newly developed gene-editing technologies using engineered CRISPR/Cas9 system and describe the application to interrogate gene functions within genetically-defined brain cell populations in sleep research.
Related Concept Videos
Cell Specific Gene Expression
Cell Specific Gene Expression
CRISPR
CRISPR/Cas9 Genome Editing
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
RNA Editing

