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Tri-ortho-cresyl phosphate (TOCP) induced ovarian failure in mice is related to the Hippo signaling pathway

Liaoliao Hu1, Tingting Peng1, Jian Huang1

  • 1Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330031, China; Jiangxi Key Laboratory of Reproductive Physiology and Pathology, Nanchang University, Nanchang, Jiangxi 330031, China.

Reproductive Toxicology (Elmsford, N.Y.)
|November 16, 2018
PubMed

Insights

Tri-ortho-cresyl phosphate (TOCP) impairs ovarian function and alters the Hippo pathway in mice. Overexpressing YAP1 partially protected against TOCP-induced ovarian damage, suggesting pathway involvement.

Area of Science:

  • Reproductive Toxicology
  • Cell Signaling
  • Developmental Biology

Background:

  • Tri-ortho-cresyl phosphate (TOCP) is a widely used plasticizer with known adverse effects on male reproduction.
  • The impact of TOCP on the female reproductive system, particularly ovarian follicle development, remains largely unexplored.
  • The Hippo pathway is a critical regulator of organ size and cellular processes, including ovarian follicle development.

Purpose of the Study:

  • To investigate the effects of chronic TOCP exposure on ovarian follicle development in a mouse model.
  • To examine the role of the Hippo pathway in TOCP-induced ovarian dysfunction.
  • To evaluate the potential protective effects of YAP1 (Yes-associated protein 1) overexpression against TOCP toxicity in the ovary.

Main Methods:

  • Chronic exposure of mice to varying doses of TOCP.
  • Assessment of ovarian function and follicle development.
  • Analysis of Hippo pathway component expression in ovarian tissues.
  • Overexpression of YAP1 in the ovaries to assess protective effects.

Main Results:

  • TOCP exposure led to a dose-dependent impairment of ovarian function.
  • Significant alterations in the expression of Hippo pathway components were observed in TOCP-exposed ovaries.
  • Overexpression of YAP1 partially ameliorated the ovarian dysfunction induced by TOCP.

Conclusions:

  • The Hippo pathway is implicated in the mechanism underlying TOCP-induced ovarian impairment.
  • TOCP poses a risk to female reproductive health, affecting ovarian follicle development.
  • YAP1 may play a protective role in mitigating TOCP's adverse effects on the ovary.
Keywords:
Hippo pathwayOvarian functionPlasticizerReproductive toxicityTri-ortho-cresyl phosphate

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