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![Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F59399.jpg&w=3840&q=50)
Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate
Published on: September 13, 2019
Efficient and accurate determination of 13C T1 and T1ρ relaxation time constants of high-mobility polymers from
Kosuke Ohgo1, Kristin K Kumashiro1
1Department of Chemistry, University of Hawaii, 2545 McCarthy Mall, Honolulu, HI 96822, USA.
Abstract:
The pulse sequences for the measurement of 13C T1 and T1ρ relaxation time constants in soft organic solids were modified to include steady-state nuclear Overhauser enhancement with direct polarization (ssNOE/DP). The increased signal intensities with ssNOE are particularly well-suited for highly mobile and structurally heterogeneous polymers and proteins like elastin. The phase cycling of these experiments was modified to yield datasets that require less time, with more accurate results. The "3D-fitting process" was developed and then optimized for natural-abundance 13C spectra of elastin, with its characteristic and significant overlap. A comparison of 3D-fitting with the similarly purposed SPORT (Geppi and Forte, 1999) illustrates that the former is more robust, with smaller uncertainty values and higher precision.
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