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Learning to draw Fischer projections of molecules and understanding their relevance plays a crucial role in the visual depiction of organic molecules. A Fischer projection is a two-dimensional projection on a planar surface to simplify the three-dimensional wedge–dash representation of molecules. This is especially helpful in the case of molecules with multiple chiral centers that can be difficult to draw. Here, all the bonds of interest are represented as horizontal or vertical lines. While...

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Related Experiment Video

Updated: Feb 2, 2026

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

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Slice-illuminated optical projection tomography.

Samuel P X Davis, Laura Wisniewski, Sunil Kumar

|November 16, 2018

Summary

This summary is machine-generated.

We developed slice-illuminated optical projection tomography (sl-OPT) to enhance live sample imaging by reducing scattered light. This method improves 3D reconstruction quality, even in scattering tissues, for applications like zebrafish imaging.

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Area of Science:

Biomedical Imaging
Optical Physics
In Vivo Microscopy

Background:

Optical Projection Tomography (OPT) is valuable for 3D imaging but struggles with scattering in live samples.
Scattered photons degrade image quality and limit resolution in traditional OPT.
Existing methods often require complex sample preparation or sacrifice imaging speed.

Purpose of the Study:

To enhance the imaging performance of OPT in live biological samples.
To reduce image artifacts caused by light scattering in thick or live tissues.
To enable high-quality 3D reconstruction of scattering samples using a modified OPT technique.

Main Methods:

Implemented a parallelized semi-confocal line illumination and detection strategy for OPT.
Utilized slice-illuminated OPT (sl-OPT) to minimize interpixel crosstalk and photon scattering.
Applied compressed sensing techniques to angularly undersampled sl-OPT data for faster acquisition in live imaging.

Main Results:

Demonstrated improved reconstruction quality in scattering samples compared to conventional OPT.
Successfully imaged 3D bead phantoms with enhanced clarity.
Acquired high-resolution 3D images of live adult zebrafish, showcasing the technique's in vivo applicability.

Conclusions:

Slice-illuminated OPT (sl-OPT) effectively discriminates against scattered photons, improving imaging in scattering media.
Compressed sensing combined with sl-OPT offers a viable approach for efficient in vivo 3D imaging.
This optimized OPT method holds promise for advanced live sample analysis in biological research.