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Updated: Feb 2, 2026

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions
Published on: October 10, 2016
Over 1000-fold improvement in an online preconcentration of trace anionic compounds by capillary electrophoresis with
Chu Chu1, Mengmeng Wei1, Caijing Liu1
1College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, PR China.
Abstract:
A sensitive, simple, and environmentally friendly online three-step stacking strategy combining field-enhanced sample injection, sweeping and micelle to solvent stacking has been developed to determine trace amounts of organic anionic analytes in complex biological samples. A green ionic liquid, 1-dodecyl-3-methylimidazolium hydrogen sulfate, was first introduced as a micellar solution and used for electroosmotic flow reversal in this stacking strategy. The mechanism of stacking has been discussed, and parameters affecting three-step stacking preconcentration efficiency have been optimized. The capillary coated with an ionic liquid was easy to prepare, regenerable and repeatable (RSD<2.16%) and yielded a high efficiency. Under optimal conditions, the sensitivity improvement increased up to 2424-fold when compared with the normal capillary zone electrophoresis mode. The peak shape and separation efficiency of the model basic organic acids showed a significant enhancement when compared to traditional capillary zone electrophoresis. The optimized method showed great potential for quantitative analysis of trace concentration analytes in complex matrices.
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