Related Experiment Video

Updated: Feb 2, 2026

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
08:27

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Published on: April 16, 2014

16.4K

miR-186-5p Promotes Apoptosis by Targeting IGF-1 in SH-SY5Y OGD/R Model

Rui Wang1, Hongbo Bao2, Shihua Zhang3

  • 1Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.

International Journal of Biological Sciences
|November 17, 2018
PubMed

Insights

MicroRNA-186-5p is elevated in ischemic stroke, promoting neuron death and reducing insulin-like growth factor 1. This microRNA may serve as a diagnostic marker and therapeutic target for stroke.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs are increasingly recognized for their roles in ischemic stroke.
  • Understanding specific microRNA functions is crucial for developing new therapeutic strategies.

Purpose of the Study:

  • To investigate the expression and function of microRNA (miR)-186-5p in ischemic stroke.
  • To elucidate the molecular mechanism underlying miR-186-5p's role in stroke.
  • To assess miR-186-5p as a potential biomarker and therapeutic target.

Main Methods:

  • Established an in vitro oxygen and glucose deprivation/reperfusion (OGD/R) model.
  • Quantified miR-186-5p expression levels.
  • Assessed the impact of miR-186-5p on neuronal apoptosis and cell viability.
  • Measured insulin-like growth factor (IGF)-1 expression.
  • Utilized a luciferase activity assay to confirm direct targeting of IGF-1 by miR-186-5p.
  • Analyzed miR-186-5p levels in serum from ischemic stroke patients and healthy donors.

Main Results:

  • miR-186-5p was significantly upregulated in the OGD/R model and induced neuronal apoptosis.
  • miR-186-5p suppressed the expression of IGF-1, a key factor for nervous system development.
  • Inhibition of miR-186-5p enhanced cell viability and IGF-1 expression.
  • IGF-1 was confirmed as a direct target of miR-186-5p.
  • miR-186-5p levels were elevated in the serum of ischemic stroke patients compared to healthy individuals.

Conclusions:

  • miR-186-5p acts as an adverse factor in ischemic stroke by promoting neuron apoptosis and inhibiting IGF-1.
  • miR-186-5p shows potential as a diagnostic marker for ischemic stroke.
  • miR-186-5p represents a promising therapeutic target for ischemic stroke patients.
Keywords:
IGF-1apoptosisischemia strokemiR-186-5p

More Related Videos

Mouse In Vivo Placental Targeted CRISPR Manipulation07:39

Mouse In Vivo Placental Targeted CRISPR Manipulation

Published on: April 14, 2023

3.7K
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells09:58

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Published on: May 2, 2017

8.1K

Related Concept Videos

Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
14.5K
Models of Health Promotion and Illness Prevention II01:18

Models of Health Promotion and Illness Prevention II

The person's health status fluctuates continually, varying from being in good health to becoming ill and returning to being healthy. To understand the concept of illness prevention, there are two models. First, the health-illness continuum model is a graphic representation of an individual's wellness. It states that a person is considered healthy in the absence of physical disease and the presence of good emotional health.
The agent-host-environment model states that disease results...
2.1K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.8K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

3.9K
Models of Health Promotion and Illness Prevention I01:25

Models of Health Promotion and Illness Prevention I

A model is a theoretical way to understand a concept or an idea. Models can overcome barriers to health regardless of diverse economic and cultural backgrounds. In addition, models make the task easier by providing different ways to approach complex issues. There are two major health promotion models: the health belief model and the health promotion model.
The health belief model (HBM) attempts to predict health-related behavior in specific belief patterns. According to the HBM, a person's...
2.8K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.4K