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Updated: Feb 2, 2026

Passaging Human Neural Stem Cells
Published on: August 22, 2007
Phenotypic stability of the human MG-63 osteoblastic cell line at different passages
Susanne Staehlke1, Henrike Rebl1, Barbara Nebe1
1Department of Cell Biology, University Medical Center Rostock, Rostock, Germany.
Abstract:
One of the most popular cell lines in osteogenesis studies is the human osteoblastic line MG-63. For cell biological investigation, it is important that the cells remain stable in their phenotype over several passages in cell culture. MG-63 cells can be used to provide fundamental insights into cell--material interaction. The aim of this study is to present a systematic characterization of the physiological behavior of MG-63 cells in the range of passages 5-30. Significant cell physiology processes during the first 24 h, including cell morphology, availability of adhesion receptors, cell cycle phases, as well as the expression of the signaling proteins Akt, GSK3a/b, IkB-α, ERK1/2, p38-MAPK, and intracellular calcium ion mobilization, remained stable over the entire range of passages P5-P30. Due to these stable characteristics in a wide range of cell culture passages, MG-63 cells can be considered as a suitable in vitro model to analyze the biocompatibility and biofunctionality of implant materials.
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