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Updated: Feb 2, 2026

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
Published on: March 2, 2011
Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary
Yong Da Sie1, Chia-Yuan Chang2, Chun-Yu Lin2
1National Cheng Kung University, Department of Engineering Science, Tainan, Taiwan.
Abstract:
Conventional temporal focusing-based multiphoton excitation microscopy (TFMPEM) can offer widefield optical sectioning with an axial excitation confinement of a few microns. To improve the axial confinement of TFMPEM, a binary computer-generated Fourier hologram (CGFH) via a digital-micromirror-device (DMD) was implemented to intrinsically improve the axial confinement by filling the back-focal aperture of the objective lens. Experimental results show that the excitation focal volume can be condensed and the axial confinement improved about 24% according to the DMD holography. In addition, pseudouniform MPE can be achieved using two complementary CGFHs with rapid pulse-width modulation switching via the DMD. Furthermore, bioimaging of CV-1 in origin with SV40 genes-7 cells demonstrates that the TFMPEM with binary DMD holography can improve image quality by enhancing axial excitation confinement and rejecting out-of-focus excitation.
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