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Response to "Determining Allele-Specific Protein Expression (ASPE) Using a Novel Quantitative Concatamer Based

Brahim Achour¹, Amin Rostami-Hodjegan^1,2, Jill Barber¹

¹Centre for Applied Pharmacokinetic Research , University of Manchester , Stopford Building, Oxford Road , Manchester M13 9PT , U.K.

Journal of Proteome Research

|November 17, 2018

Summary

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Quantitative proteomic methods using QconCAT standards for allele-specific expression analysis are not novel. Our group previously applied this technique to quantify CYP2B6 alleles, demonstrating its utility for polymorphism assessment.

Area of Science:

Proteomics
Pharmacogenomics
Molecular Biology

Background:

A recent publication utilized quantitative proteomic strategy with a quantitative concatemer (QconCAT) standard for allele-specific expression assessment of UGT2B15.
The publication claimed this methodology as a "novel" approach.

Purpose of the Study:

To address the claim of novelty regarding the QconCAT standard application in allele-specific expression analysis.
To highlight prior work by the authors using the same methodology.

Main Methods:

Targeted proteomic strategy employing a quantitative concatemer (QconCAT) standard.
Quantification of allele-specific expression.

Main Results:

The QconCAT standard methodology for allele-specific expression analysis was previously reported by our group.

Related Experiment Videos

Our prior work focused on the quantification of CYP2B6 alleles to assess a prevalent polymorphism in a Caucasian population.

Conclusions:

The application of QconCAT standards for allele-specific expression analysis, while not common, has been previously described.
The methodology is a valid approach for assessing allele-specific expression and polymorphisms.