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The rebound hammer test, also known as the Schmidt hammer test, is a non-destructive technique for evaluating the hardness of concrete and, indirectly, the strength of concrete. It operates on the principle that the rebound of a spring-driven mass from a concrete surface correlates to the surface's hardness. The device comprises a mass within a tubular housing, a spring mechanism, and a plunger that strikes the concrete. Upon release, the energy imparted to the mass by the spring causes it...
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Building stones, essential materials for construction, are extracted from natural rock deposits and processed into specific forms and dimensions suitable for various building applications. These stones are broadly classified into three types based on their geological formation: igneous, sedimentary, and metamorphic.
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  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

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|November 17, 2018
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Summary
This summary is machine-generated.

p97 ATPase, with a SEP adaptor, drives protein phosphatase-1 (PP1) holoenzyme assembly. This study reveals a new ubiquitin-independent "segregase" function for p97 in protein biogenesis.

Keywords:
AAA ATPasePP1SEPp37p97/VCP/CDC48protein phosphatase 1ubiquitin

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Protein phosphatase-1 (PP1) is crucial for cellular regulation.
  • PP1 holoenzyme biogenesis requires precise assembly of its subunits.
  • The AAA+ ATPase p97 is involved in various cellular processes, including protein complex remodeling.

Purpose of the Study:

  • To investigate the role of p97 and its adaptors in PP1 holoenzyme formation.
  • To elucidate the mechanism by which p97 facilitates PP1 assembly.
  • To identify novel functions of p97 beyond its known roles.

Main Methods:

  • Biochemical assays to study p97 ATPase activity.
  • In vitro reconstitution of PP1 holoenzyme assembly.
  • Analysis of protein-protein interactions using co-immunoprecipitation.

Main Results:

  • p97, in complex with the SEP adaptor, catalyzes ordered subunit exchange.
  • This process is essential for the efficient biogenesis of the PP1 holoenzyme.
  • p97 functions as a ubiquitin-independent "segregase" in this context.

Conclusions:

  • p97 and SEP adaptor collaborate to ensure proper PP1 holoenzyme assembly.
  • A novel ubiquitin-independent segregase function of p97 is demonstrated.
  • This finding expands our understanding of p97's role in protein complex biogenesis.