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Updated: Feb 2, 2026

Microhoneycomb Monoliths Prepared by the Unidirectional Freeze-drying of Cellulose Nanofiber Based Sols: Method and Extensions
Published on: May 24, 2018
Preparation of a hydroxyethyl-based monolithic column and its application in the isolation of intact proteins from
Dandan Lan1, Ligai Bai1, Mingxue Li1
1College of Pharmaceutical Sciences, Hebei University, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, China; Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding 071002, China.
Abstract:
In this work, a monolithic hydroxyethyl-based column was fabricated in a stainless-steel column (50 mm × 4.6 mm i.d.) via radical polymerization technique using hydroxyethyl methylacrylate as the monomer. The morphology and pore size distribution indicate that the optimized monolith has a relatively uniform structure with macro-pores. The homemade monolith was used as the stationary phase of high performance liquid chromatography for the separation of intact proteins from complex bio-samples, including human plasma, egg white and snailase. The resulting monolith shows excellent selectivity for intact proteins mainly depending on the different relative hydrophobicity of the objective proteins with reversed-phase liquid chromatographic mechanism. Besides, the hydrogen-bond interaction and electrostatic interaction were the additional interactions in the chromatographic separation owing to hydroxyl groups present in the surface of monolithic material.
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