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Tumor-Targeted Immunotherapy by Using Primary Adipose-Derived Stem Cells and an Antigen-Specific Protein Vaccine

Jui-Hua Lu1,2, Bou-Yue Peng3,4, Chun-Chao Chang5,6

  • 1Graduate Institute of Biomedical Materials and Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan. d225101001@tmu.edu.tw.

Cancers
|November 18, 2018
PubMed

Insights

This study presents a novel cancer immunotherapy using modified adipose-derived stem cells (ADSCs) and a protein vaccine. The enhanced platform demonstrated significant antitumor effects by inducing apoptosis and inhibiting angiogenesis in mouse models.

Area of Science:

  • Oncology
  • Immunotherapy
  • Stem Cell Biology

Background:

  • Cancer remains a significant global health challenge, driving the need for innovative therapeutic strategies.
  • Previous immunotherapeutic platforms combining mesenchymal stem cells (MSCs) with protein vaccines faced limitations, including issues with MSC immortalization and oncogenic antigen transfection.
  • Overcoming these limitations is crucial for advancing biological therapies toward clinical application.

Purpose of the Study:

  • To develop an improved immunotherapeutic platform for cancer treatment by utilizing primary adipose-derived stem cells (ADSCs) and a modified non-oncogenic antigen (E7').
  • To evaluate the antitumor efficacy and underlying mechanisms of the novel ADSC-based immunotherapy in preclinical cancer models.

Main Methods:

  • Primary ADSCs were prepared and modified with enhanced green fluorescent protein (eGFP) and a non-oncogenic E7' antigen (ADSC-E7'-eGFP).
  • The therapeutic efficacy was assessed in mouse models of colon and lung cancer, with administration either subcutaneously or systemically.
  • Tumor inhibition, apoptosis, angiogenesis markers (CD31, VEGF), and immune cell responses (CD4+ T, NK, CD8+ T cells) were analyzed.

Main Results:

  • ADSC-E7'-eGFP combined with a protein vaccine demonstrated significant antitumor activity in both colon and lung cancer models.
  • The therapy effectively induced cancer cell apoptosis and markedly inhibited tumor angiogenesis, evidenced by reduced CD31 and VEGF levels.
  • Immune system activation was observed, including responses from CD4+ T cells, natural killer (NK) cells, and potentially CD8+ T cells.

Conclusions:

  • The novel ADSC-E7'-eGFP immunotherapeutic platform shows promising preclinical efficacy against cancer.
  • This enhanced platform overcomes previous limitations and warrants further investigation for potential clinical application in cancer treatment.
Keywords:
T cell responsesadipose-derived stem cellsantigen processingprotein vaccinetumor microenvironment

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