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Related Concept Videos

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Facilitated Transport

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The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
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The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
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Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
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The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Related Experiment Video

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Amplicon Sequencing using the Long-Read Sequencing Technologies
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Published on: August 29, 2025

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Single-molecule long-read sequencing facilitates shrimp transcriptome research.

Digang Zeng1, Xiuli Chen1, Jinxia Peng1

  • 1Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning, Guangxi, P.R. China.

Scientific Reports
|November 18, 2018
PubMed
Summary
This summary is machine-generated.

This study generated over 51,000 full-length transcripts for the Pacific white shrimp (Litopenaeus vannamei) using long-read sequencing. This valuable resource will advance shrimp transcriptome research and genetic studies.

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Area of Science:

  • Marine Biology
  • Genomics
  • Biotechnology

Background:

  • Shrimp hold significant economic value, yet comprehensive transcriptome data is limited.
  • Understanding shrimp gene expression is crucial for aquaculture and fisheries.

Purpose of the Study:

  • To generate a high-quality, full-length transcriptome dataset for the Pacific white shrimp (Litopenaeus vannamei).
  • To provide a foundational resource for future research in shrimp genomics and molecular biology.

Main Methods:

  • Utilized Pacific Biosciences single-molecule real-time (SMRT) long-read sequencing technology.
  • Generated and curated 51,367 unique full-length transcripts.
  • Performed error correction using Illumina short reads and extensive database annotations.

Main Results:

  • Successfully annotated a high percentage of transcripts against major biological databases (NCBI, Swiss-Prot, GO, COG, KEGG).
  • Identified 3,958 long non-coding RNAs (lncRNAs) and 80,650 simple sequence repeats (SSRs).
  • Produced a comprehensive set of full-length cDNA sequences for L. vannamei.

Conclusions:

  • The generated transcriptome dataset is a valuable resource for L. vannamei research.
  • Facilitates advancements in shrimp aquaculture, disease resistance, and genetic improvement.
  • Enables deeper insights into shrimp molecular mechanisms and evolutionary biology.