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Interleukin-33 gene expression and rs1342326 polymorphism in Behçet's disease
Mahsa Talei1, Ali Abdi2, Dariush Shanebandi3
1Connective Tissue Research Center, Tabriz University of Medical Science, Iran; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Immunology Letters
|November 18, 2018
Summary
Genetic variations in the Interleukin-33 (IL-33) gene, specifically the rs1342326 T/G polymorphism, are linked to increased susceptibility to Behçet's disease (BD). This polymorphism correlates with higher IL-33 gene expression in patients with BD.
Area of Science:
- Immunogenetics
- Molecular Biology
- Rheumatology
Background:
- Behçet's disease (BD) is a chronic inflammatory condition with a significant genetic component.
- Interleukin-33 (IL-33) is a cytokine involved in immune responses and inflammation.
Purpose of the Study:
- To investigate the association between the IL-33 gene rs1342326 polymorphism and its expression levels in peripheral blood mononuclear cells (PBMCs) with BD susceptibility in the Azari population of Iran.
Main Methods:
- A cross-sectional study involving 44 BD patients and 61 healthy controls.
- Genotyping of the IL-33 rs1342326 single nucleotide polymorphism using Tetra-ARMS-PCR.
- Quantification of IL-33 mRNA levels in PBMCs via Real-time PCR.
Main Results:
- A significantly higher prevalence of the rs1342326 T/G genotype was observed in BD patients compared to controls.
- IL-33 expression levels in PBMCs were significantly elevated in the BD group.
- The rs1342326 T/G polymorphism was associated with increased IL-33 expression in BD patients.
Conclusions:
- The rs1342326 T/G polymorphism in the IL-33 gene may contribute to genetic susceptibility to Behçet's disease.
- This contribution appears to be mediated, in part, by the regulation of IL-33 expression.