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Updated: Feb 2, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Structural characterization, hypoglycemic effects and mechanism of a novel polysaccharide from Tetrastigma
Yi Ru1, Xiao Chen1, Jian Wang1
1Ministry of Education Key Laboratory of Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350108, PR China.
Abstract:
In the present study, a novel polysaccharide (THDP-3) purified from cane leaves of Tetrastigma hemsleyanum Diels et Gilg was obtained via anion exchange chromatography and the structural analyses of the compound indicated that THDP-3 is a homogenous polysaccharide with molecular weight of 77.98 kDa. Our results show that it consists of rhamnose, arabinose, mannose, glucose and galactose at molar ratio of 1.0: 1.3: 2.5: 2.3:3.1 with main backbones of →4)-α-D-GalAp-(1→, →4)-β-D-Galp-(1→ and →4)-α-D-Glcp-(1→, and main branches of β-D-Manp-(1→, →3,6-β-D-Manp-1→ and α-D-Araf-(1→. In the anti-diabetic assays, THDP-3 showed significant hypoglycemic activity in alloxan-induced diabetic mice. In addition, THDP-3 was found regulating the expression of glucokinase, amp-activated protein kinase, glucose-6-phosphatase and phosphoenolpyruvate carboxykinase. These new findings indicate the high potential in therapeutic mechanisms investigation using THDP-3. Moreover, our results of hypoglycemic activity using THDP-3 demonstrated that the compound could serve as a potential anti-diabetic agent.
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