Related Experiment Video

Updated: Feb 2, 2026

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
05:49

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

1.2K

Phosphatidylserine expressing microvesicles in relation to microvascular complications in type 1 diabetes

Karin Bergen1, Fariborz Mobarrez2, Gun Jörneskog3

  • 1Karolinska Institutet, Department of Clinical Sciences, Danderyd University Hospital, Division of Nephrology, Stockholm, Sweden.

Thrombosis Research
|November 18, 2018
PubMed
Abstract

Insights

Patients with type 1 diabetes show increased microvesicles (MVs) and lactadherin, a clearance glycoprotein. While MVs are elevated, lactadherin levels correlate with microangiopathy, suggesting its role in vascular complications.

Area of Science:

  • Endocrinology
  • Vascular Biology
  • Hematology

Background:

  • Type 1 diabetes is a prothrombotic condition associated with vascular complications.
  • The specific role of microvesicles (MVs) in type 1 diabetes-related microangiopathy is not fully understood.

Purpose of the Study:

  • To investigate microvesicle (MV) levels and their association with microangiopathy in type 1 diabetes.
  • To explore the role of lactadherin in type 1 diabetes and its potential link to microangiopathy.

Main Methods:

  • Flow cytometry was used to analyze MV levels in plasma from type 1 diabetes patients and healthy controls.
  • Phosphatidylserine (PS) expression on MVs and their thrombin generation capacity were assessed.
  • ELISA was employed to measure endogenous plasma lactadherin levels.

Main Results:

  • Patients with type 1 diabetes exhibited higher circulating MV levels than controls, irrespective of microangiopathy status.
  • MV-induced thrombin generation was inhibited by lactadherin.
  • Plasma lactadherin levels were elevated in type 1 diabetes patients, particularly those with microangiopathy, but did not correlate with PS-positive MVs.

Conclusions:

  • Elevated MV levels in type 1 diabetes suggest increased cellular activation, but do not directly correlate with clinical microangiopathy.
  • Increased plasma lactadherin levels in type 1 diabetes patients correlate with microangiopathy, indicating its potential involvement in the condition's vascular complications.
Keywords:
LactadherinMicroangiopathyMicroparticlesMicrovesiclesType 1 diabetes

More Related Videos

Transient Expression in Red Beet of a Biopharmaceutical Candidate Vaccine for Type-1 Diabetes10:16

Transient Expression in Red Beet of a Biopharmaceutical Candidate Vaccine for Type-1 Diabetes

Published on: March 19, 2019

8.4K
A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes05:18

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

Published on: February 17, 2023

5.8K

Related Concept Videos

Diabetes: Symptoms, Diagnosis, and Complications01:15

Diabetes: Symptoms, Diagnosis, and Complications

For most patients, experiencing several weeks of polyuria, polydipsia, fatigue, and significant weight loss may indicate the presence of diabetes. Furthermore, adults displaying the phenotypic appearance of type 2 diabetes (particularly those who are obese and not initially insulin-requiring), may have islet cell autoantibodies, suggesting autoimmune-mediated β cell destruction and a diagnosis of latent autoimmune diabetes of adults (LADA). The categorization of glucose homeostasis is...
2.3K
Diabetes Mellitus: Type 2 and Gestational01:22

Diabetes Mellitus: Type 2 and Gestational

Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
5.0K
Diabetes Mellitus: Overview and Type I Subtype01:22

Diabetes Mellitus: Overview and Type I Subtype

Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
5.4K
Hemodialysis II: Procedure and Complications01:24

Hemodialysis II: Procedure and Complications

DialyzersA hemodialysis (HD) dialyzer is a plastic cartridge containing thousands of parallel hollow fibers, which serve as semipermeable membranes. These fibers are typically made from cellulose-based or other synthetic materials. During HD, blood is pumped into the top of the cartridge and distributed among these fibers. Simultaneously, dialysis fluid, known as dialysate, is introduced into the bottom of the cartridge, bathing the outside of the fibers. Across the semipermeable membrane,...
821
Pathophysiology of Diabetes01:20

Pathophysiology of Diabetes

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
3.6K
Pneumonia III: Complications and Assessment01:30

Pneumonia III: Complications and Assessment

Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
861