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Updated: Feb 2, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
Forensic tri-allelic SNP genotyping using nanopore sequencing
Senne Cornelis1, Yannick Gansemans2, Ann-Sophie Vander Plaetsen2
1Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000 Gent, Belgium; Department of Life Sciences and Imaging, imec, 3001 Leuven, Belgium.
Abstract:
The potential and current state-of-the-art of forensic SNP genotyping using nanopore sequencing was investigated with a panel of 16 tri-allelic single nucleotide polymorphisms (SNPs), multiplexing five samples per sequencing run. The sample set consisted of three single-source human genomic reference control DNA samples and two GEDNAP samples, simulating casework samples. The primers for the multiplex SNP-loci PCR were taken from a study which researched their value in a forensic setting using conventional single-base extension technology. Workflows for multiplexed Oxford Nanopore Technologies' 1D and 1D2 sequencing were developed that provide correct genotyping of most SNP loci. Loci that are problematic for nanopore sequencing were characterized. When such loci are avoided, nanopore sequencing of forensic tri-allelic SNPs is technically feasible.
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